Chromosomal DNA Analyses of Staphylococci

  • Fiona M. Thomson-Carter
  • T. Hugh Pennington

Summary

Ribosomal RNA (rRNA) gene restriction fragment length polymorphisms and pulsed field gel electrophoresis (PFGE) have been used in analyses of staphylococcal strains and species. Analyses of DNA from different species of staphylococci using a radioactively labelled rRNA probe gave reproducible rRNA gene restriction patterns. These were species-specific, different strains of a particular species having identical or similar profiles. PFGE can also be used to generate restriction profiles which are reproducible and characteristic for different species. Restriction profiles obtained by these methods could be exploited as taxonomic criteria.

Keywords

Bromide Electrophoresis Polypeptide Pseudomonas Ethidium 

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Copyright information

© Springer-Verlag London Limited 1990

Authors and Affiliations

  • Fiona M. Thomson-Carter
  • T. Hugh Pennington

There are no affiliations available

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