Infertility pp 101-114 | Cite as

Sperm Preservation: Fundamental Cryobiology and Practical Implications

  • P. F. Watson
  • J. K. Critser
  • P. Mazur

Abstract

Human spermatozoa were first frozen successfully almost 40 years ago [1]. Although developments in dilution media, packaging and storing have occurred in the intervening period, little is known specifically about the effects of freezing and thawing on spermatozoa. This is largely due to the fact that a sufficient number of cells survive the challenge to give a reasonable expectation of fertilisation; but the shift to the exclusive use of cryopreserved semen for donor insemination, occasioned by the awareness of the risks particularly of AIDS transmission [2–4], has resulted in a renewed critical interest in the process of cryopreservation. This review will cover the more significant contributions over the past few years, and develop an argument for a sustained fundamental approach to sperm cryobiology.

Keywords

Permeability Sucrose Glycerol Depression Shrinkage 

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Copyright information

© Royal College of Obstetricians and Gynaecologists 1992

Authors and Affiliations

  • P. F. Watson
  • J. K. Critser
  • P. Mazur

There are no affiliations available

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