Infertility pp 253-261 | Cite as

Cryopreservation of Mammalian Oocytes

  • D. G. Whittingham
  • J. G. Carroll


A mature viable oocyte is central to the success of in vitro fertilisation (IVF) programmes and other reproductive technologies. The ability to cryopreserve the oocyte would provide a means of banking oocytes for patients where loss of gonadal function is anticipated. The main clinical indications for oocyte storage are:
  1. 1.

    Donation to individuals lacking ovaries or with ovaries devoid of oocytes, e.g. premature menopause

  2. 2.

    Donation to carriers of severe genetic diseases

  3. 3.

    Prior to chemo- or radiotherapy treatment

  4. 4.

    Prior to oophorectomy

  5. 5.

    To avoid producing excess embryos in IVF treatment

  6. 6.

    Oocytes in excess of those transferred in a GIFT treatment.



Zona Pellucida Mouse Oocyte Human Oocyte Cortical Granule Meiotic Spindle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Whittingham DG. Fertilization in vitro and development to term of unfertilized mouse oocytes previous stored at -196°C. J Reprod Fertil 1977; 49: 89–94.PubMedCrossRefGoogle Scholar
  2. 2.
    Whittingham DG, Leibo SP, Mazur P. Survival of mouse embryos frozen to -196°C and -269°C. Science 1972; 178: 411–14.PubMedCrossRefGoogle Scholar
  3. 3.
    Wilmut I. Effect of cooling rate, warming rate, cryoprotective agent and stage of development on survival of mouse embryos during freezing and thawing. Life Sci 1972; 11: 1071–9.CrossRefGoogle Scholar
  4. 4.
    Whittingham DG, Wood M, Farrant J, Lee H, Halsey JA. Survival of frozen mouse embryos after rapid thawing from -196°C. J Reprod Fertil 1979; 56: 11–21.PubMedCrossRefGoogle Scholar
  5. 5.
    Sze11 A, Shelton JN. Osmotic and cryoprotective effects of glycerol-sucrose solutions on day-3 mouse embryos. J Reprod Fertil 1987; 80: 309–16.PubMedCrossRefGoogle Scholar
  6. 6.
    Trounson A, Peura A, Kirby C. Ultrarapid freezing: a new low-cost and effective method of embryo cryopreservation. Fertil Steril 1987; 48: 843–50.PubMedGoogle Scholar
  7. 7.
    Rall WF, Fahy GM. Ice-free cryopreservation of mouse embryos at -196°C by vitrification. Nature 1984; 313: 573–5.CrossRefGoogle Scholar
  8. 8.
    Glenister PH, Wood MJ, Kirby C, Whittingham DG. Incidence of chromosome anomalies in first-cleavage mouse embryos obtained from frozen-thawed oocytes fertilized in vitro. Gamete Res 1987; 16: 205–16.PubMedCrossRefGoogle Scholar
  9. 8.
    Al-Hasani S, Kirsch J, Diedrich K, Blanke S, van der Ven H, Krebs D. Successful embryo transfer of cryopreserved and in-vitro fertilized rabbit oocytes. Hum Reprod 1989; 4: 77–9.PubMedGoogle Scholar
  10. 10.
    Vincent C, Gamier V, Heyman Y, Renard JP. Solvent effects on cytoskeletal organization and in-vivo survival after freezing of rabbit oocytes. J Reprod Fertil 1989; 87: 809–20.PubMedCrossRefGoogle Scholar
  11. 11.
    Chen C. Pregnancy after human oocyte cryopreservation. Lancet 1986; 1: 884–6.PubMedCrossRefGoogle Scholar
  12. 12.
    Van Uem JFHM, Siebzehnrubl ER, Schuh B, Koch R, Trotnow S, Lang N. Birth after cryopreservation of unfertilized oocytes. Lancet 1987; 1: 752–3.PubMedGoogle Scholar
  13. 13.
    Trounson AO, Mohr L. Human pregnancy following cryopreservation thawing and transfer of an eight-cell embryo. Nature 1983; 305: 707–9.PubMedCrossRefGoogle Scholar
  14. 14.
    Zeilmaker GH, Alberda AT, Van Gent I, Rijkmans CMPM, Drogendijk AC. Two pregnancies following transfer of intact frozen-thawed embryos. Fertil Steril 1984; 42: 293–6.PubMedGoogle Scholar
  15. 15.
    Al-Hasani S, Diedrich K, van der VEN H, Reinecke A, Hartje M, Krebs D. Cryopreservation of human oocytes. Hum Reprod 1987; 2: 695–700.PubMedGoogle Scholar
  16. 16.
    Sathananthan AH, Trounson A, Freeman L. Morphology and fertilizability of frozen human oocytes. Gamete Res 1987; 16: 343–54.PubMedCrossRefGoogle Scholar
  17. 17.
    Mandelbaum J, Junca AM, Tib C et al. Cryopreservation of immature and mature hamster and human oocytes. Ann NY Acad Sci 1988; 541: 550–61.PubMedCrossRefGoogle Scholar
  18. 18.
    Mazur P, Schneider U. Osmotic responses of preimplantation mouse and bovine embryos and their cryobiological implications. Cell Biophys 1986; 8: 259–86.PubMedGoogle Scholar
  19. 19.
    Hunter JE, Bernard B, Fuller B, Amso N, Shaw RW. Fertilisation and development of the human oocyte following exposure to cryoprotectants, low temperatures and cryopreservation: a comparison of two techniques. Hum Reprod 1991; 6: 1460–5.PubMedGoogle Scholar
  20. 20.
    Bouquet M, Selva J, Auroux M. The incidence of chromosomal abnormalities in frozen-thawed mouse oocytes after in vitro fertilization. Hum Reprod 1992; 7: 76–80.PubMedGoogle Scholar
  21. 21.
    Kola I, Kirby C, Shaw J, Davey A, Trounson A. Vitrification of mouse oocytes results in aneuploid zygotes and malformed fetuses. Teratology 1988; 38: 467–74.PubMedCrossRefGoogle Scholar
  22. 22.
    Bos-Mikich A, Whittingham DG. Parthenogenetic activation of frozen-thawed mouse oocytes. J Reprod Fertil Abstr Ser 1991; 7: 18.Google Scholar
  23. 23.
    Algaier J, Himes RH. The effects of dimethylsulfoxide on the kinetics of tubulin assembly. Biochim Biophys Acta 1988; 954: 235–43.PubMedCrossRefGoogle Scholar
  24. 24.
    Himes RH, Burton P, Gaito JM. Dimethylsulfoxide-induced self-assembly of tubulin lacking associated proteins. J Biol Chem 1977; 252: 6222–8.PubMedGoogle Scholar
  25. 25.
    Pickering SJ, Johnson MH, Braude PR, Houliston E. Cytoskeletal organization, in fresh, aged and spontaneously activated human oocytes. Hum Reprod 1988; 3: 978–89.PubMedGoogle Scholar
  26. 26.
    Carroll J, Warnes GM, Matthews CD. Increase in digyny explains polyploidy after in-vitro fertilisation of frozen-thawed mouse oocytes. J Reprod Fertil 1989; 85: 489–94.PubMedCrossRefGoogle Scholar
  27. 27.
    Maro B, Johnson MH, Pickering SJ, Flach G. Changes in actin distribution during fertilization of the mouse egg. J Embryol Exp Morphol 1984; 81: 211–37.PubMedGoogle Scholar
  28. 28.
    Vincent D. Dimethylsulfoxide affects the organization of microfilaments in the mouse oocyte. Mol Reprod 1990; 26: 227–35.CrossRefGoogle Scholar
  29. 29.
    Vincent C, Pruliere G, Pajot-Augy E, Campion E, Gamier V, Renard JP. Effects of cryoprotectants on actin filaments during the cryopreservation of one-cell rabbit embryos. Cryobiology 1990; 27: 9–23.PubMedCrossRefGoogle Scholar
  30. 30.
    Carroll J, Depypere H, Matthews CD. Freeze-thaw induced changes of the zona pellucida explains decreased rates of fertilization in frozen-thawed mouse oocytes. J Reprod Fertil 1990; 90: 547–53.PubMedCrossRefGoogle Scholar
  31. 31.
    Wood MJ, Whittingham DG, Lee S-H. Fertilization failure of frozen mouse oocytes is not due to premature cortical granule release. Biol Reprod 1992; 46Google Scholar
  32. 32.
    Schroeder AC, Champlin AK, Mobraaten LE, Eppig JJ. Developmental capacity of mouse oocytes cryopreserved before and after maturation in vitro. J Reprod Fertil 1990; 89: 43–50.PubMedCrossRefGoogle Scholar
  33. 33.
    Wood MJ, Carroll J, Whittingham DG. The addition of serum to the medium limits zona hardening in frozen oocytes. J Reprod Fertil Abstr Ser 1989; 3: 33.Google Scholar
  34. 34.
    Downs SM, Schroeder AC, Epigg JJ. Serum maintains the fertilizability of mouse oocytes matured in vitro by preventing hardening of the zona pellucida. Gamete Res 1986; 15: 115–22.CrossRefGoogle Scholar
  35. 35.
    Choi TS, Mori M, Kohmoto K, Shoda Y. Beneficial effect of serum on the fertilizability of mouse oocytes matured in vitro. J Reprod Fertil 1987; 79: 505–8.CrossRefGoogle Scholar
  36. 36.
    Gulyas BJ, Yuan LC. Cortical reaction and zona hardening in mouse oocytes following exposure to ethanol. J Exp Zool 1985; 233: 269–76.PubMedCrossRefGoogle Scholar
  37. 37.
    Moller CC, Wassarman PM. Characterization of a proteinase that cleaves zona pellucida glycoprotein ZP2 following activation of mouse eggs. Dev Biol 1989; 132: 103–112.PubMedCrossRefGoogle Scholar
  38. 38.
    Ducibella T, Kurasawa S, Rangarajan S, Kopf GS, Schultz RM. Precocious loss of cortical granules during mouse oocyte meiotic maturation and correlation with an egg-induced modification of the zona pellucida. Dev Biol 1990; 137: 46–55.PubMedCrossRefGoogle Scholar
  39. 39.
    Vincent C, Pickering SJ, Johnson MH. The hardening effect of dimethylsulphoxide on the mouse zona pellucida requires the presence of an oocyte and is associated with a reduction in the number of cortical granules present. J Reprod Fertil 1990; 89: 253–9.PubMedCrossRefGoogle Scholar
  40. 40.
    DeFelici M, Siracusa G. “Spontaneous” hardening of the zona pellucida of mouse oocytes during in vitro culture. Gamete Res 1982; 6: 107–13.CrossRefGoogle Scholar
  41. 41.
    Freidler S, Giudice LC, Lamb EJ. Cryopreservation of embryos and ova. Fertil Steril 1988; 49: 743–64.Google Scholar
  42. 42.
    Wood MJ, Rall WF. The preservation of embryos by vitrification. Hum Reprod 1987; 2:Suppl 1, 2–3.Google Scholar
  43. 43.
    Rall WF. Factors affecting the survival of mouse embryos cryopreserved by vitrification. Cryobiology 1987; 24: 387–402.PubMedCrossRefGoogle Scholar
  44. 44.
    Trounson A. Preservation of human eggs and embryos. Fertil Steril 1986; 46: 1–12.PubMedGoogle Scholar
  45. 45.
    Bos-Mikich A, Candy C, Wood MJ, Whittingham DG. Cytogenetic analysis and developmental potential of vitrified mouse oocytes. J Reprod Fertil Abstr Ser 1992; 9Google Scholar
  46. 46.
    Edirisinghe WR, Murch AR, Yovich JL. Cytogenetic analysis of human ooctyes and embryos in an in-vitro fertilization programme. Hum Reprod 1992; 7: 230–6.PubMedGoogle Scholar
  47. 47.
    Tarin JJ, Gomez E, Sampaio M, Ruiz M, Remohi J, Pellicer A. Cytogenetic analysis of human oocytes from fertile women. Hum Reprod 1991; 6: 1100–3.PubMedGoogle Scholar
  48. 48.
    Angell RR, Ledger W, Yong EL, Harkness L, Baird DT. Cytogenetic analysis of unfertilized human oocytes. Hum Reprod 1991; 6: 568–73.PubMedGoogle Scholar

Copyright information

© Royal College of Obstetricians and Gynaecologists 1992

Authors and Affiliations

  • D. G. Whittingham
  • J. G. Carroll

There are no affiliations available

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