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Therapeutic Antibodies to Snake Venoms

Chapter

Abstract

Studies on immunization against snake venoms began over 100 years ago when Sewall (1887) demonstrated that pigeons could be immunized against the venom of the pygmy rattlesnake (Sistrurus catenatus tergeminus) after treating the animals with gradually increasing amounts of the venom. These studies were followed closely by those of Calmette (1894a, 1895, 1898) and Phisalix and Bertrand (1894) who immunized horses against snake venoms. Calmette (1894a) recommended mixtures of hypochlorite and venom, and Phisalix and Bertrand (1894) used heat-treated venom in order to reduce the toxicity and retain the antigenicity of the immunogens. Subsequently, Fraser (1895), working in Scotland, produced a specific immune serum against cobra venom. Calmette’s work resulted in the publication of a book on venoms which contained the first basic information on techniques for animal immunization against venoms. Later, Pasteur Institutes throughout the world started to produce and supply antivenoms on a commercial basis. Further detailed studies on antivenoms were made by Brazil (1911) in Sao Paulo; the Instituto Butantan subsequently initiated large-scale antivenom production, which has continued almost unchanged to the present day.

Keywords

Snake Venom Therapeutic Antibody Snake Bite Venom Component Antivenom Therapy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Adler FL (1957) Antibody formation after injection of heterologous immunoglobulin - II. Competition of antigens. J Immunol 78: 201–210PubMedGoogle Scholar
  2. Al-Joufi A, Bailey GS, Reddi K, Smith DC (1991) Neutralisation of kinin-releasing enzymes from viperid venoms by antivenom IgG fragments. Toxicon 29: 1509–1512PubMedGoogle Scholar
  3. Amies CR (1959) The use of topically formed calcium alginate as a depot substance in active immunization. J Path Bact 77: 435–442PubMedGoogle Scholar
  4. Bailey GS, Al-Joufi A, Rawat S, Smith DC (1991) Neutralisation of kinin-releasing enzymes from crotalid venoms by monospecific and polyspecific antivenoms. Toxicon 29: 777–781PubMedGoogle Scholar
  5. Benson HN, Brumfield HP et al. (1961) Requirement of avian CI for fixation of guinea pig complement by avian antibody-antigen complexes. J Immunol 87: 616–622PubMedGoogle Scholar
  6. Boulain J-C, Menez A (1982) Neurotoxin-specific immunoglobulins accelerate dissociation of the neurotoxin-acetylcholine receptor complex. Science 217: 732–733PubMedGoogle Scholar
  7. Boulain J-C, Menez A, Couderc J, Faure G, Liacopoulos P, Fromageot P (1982) Neutralizing monoclonal antibody specific for Naja nigricollis toxin: Preparation, characterization and localization of the antigenic binding site. Biochemistry 21: 2910–2915PubMedGoogle Scholar
  8. Bouquet P (1979) Immunological properties of snake venoms. In: Lee C-Y (ed) Snake venoms. Springer, Berlin, pp 751–824 (Handbook of experimental pharmacology, vol 52)Google Scholar
  9. Braun W, Nakano M (1967) Antibody formation: Stimulation by polyadenylic acids. Science 157: 819–821PubMedGoogle Scholar
  10. Brazil V (1911) La defense contre l’ophidisme. Instituto Butantan, Sao Paulo, BrazilGoogle Scholar
  11. Buchowicz I, Goch H, Zakrzewski K (1969) Equine immunoglobulins: A comparison of molecular properties. Acta Biochem Polon 16: 279–294Google Scholar
  12. Calmette LCA (1894a) Contribution a l’etude du venin des serpents (immunization des animaux et traitement de l’envenimation). Ann Inst Pasteur 8: 275–291Google Scholar
  13. Calmette LCA (1894b) Proprietes du serum des animaux immunises contre le venin des serpentes, et therapeutique de l’envenomation. C R Acad 68: 720–725Google Scholar
  14. Calmette LCA (1895) Contribution a l’etude des venins, des toxins et des serums antitoxiques. Ann Inst Pasteur 9: 225–251Google Scholar
  15. Calmette LCA (1898) Sur la mechanisme de l’ immunisation contre les venins. Ann Inst Pasteur 12: 343–353Google Scholar
  16. Cardoso JLC, Fan HW, Franca FOS et al. (1993) Randomized comparative trial of three antivenoms in the treatment of envenoming by lance-headed vipers (Bothrops jararaca) snakes in Sāo Paulo, Brazil. J Med 86: 315–325Google Scholar
  17. Carroll SB, Thalley BS, Theakston RDG, Laing G (1992) A comparison of the purity and efficacy of affinity purified avian antivenoms with commercial equine crotalid antivenoms. ToxiconGoogle Scholar
  18. Cartledge C, Maclean C, Landon J (1992) Production of polyclonal antibodies in ascitic fluid of mice - time and dose relationships. J Immunoass 13: 339–353Google Scholar
  19. Celada F (1971) The cellular basis of immunologic memory. Prog Allergy 15: 223–267PubMedGoogle Scholar
  20. Chippaux J-P, Goyffon M (1983) Producers of antivenomous sera. Toxicon 21: 739–752PubMedGoogle Scholar
  21. Chippaux J-P, Williams V, White J (1991) Snake venom variability: Methods of study, results and interpretation. Toxicon 29: 1279–1303PubMedGoogle Scholar
  22. Christensen PA (1955) “South African snake venoms and antivenoms”. The South African Institute for Medical Research, JohannesburgGoogle Scholar
  23. Christensen PA (1962) Antivenom standardization. Progress report, WHO Expert Committee on Biological Standardization, Geneva, 10–15 DecemberGoogle Scholar
  24. Christensen PA (1966a) The preparation and purification of antivenoms. Mems Inst Butantan Simp Internac 33: 245–250Google Scholar
  25. Christensen PA (1966b) Venom and antivenom potency estimation. Mems Inst Butantan 33: 305–326Google Scholar
  26. Christensen PA (1968) The venoms of Central and South African snakes. In: Bucherl W, Buckley EE, Deulofeu V (eds) Venomous animals and their venoms. Vol I, Venomous vertebrates, Ch 16. Academic Press, New York, pp 437–461Google Scholar
  27. Christensen PA (1979) Production and standardisation of antivenin. In: Lee CY (ed) Snake venoms. Springer-Verlag, Berlin, pp 825–846 (Handbook of experimental pharmacology, vol 52)Google Scholar
  28. Corrigan P, Russell FE, Wainschel J Jr (1978) Clinical reactions to antivenin. In: Rosenberg P (ed) Toxins: Animal, plant, microbial. Pergamon Press, OxfordGoogle Scholar
  29. Coulter AR, Cox JC, Sutherland SK, Waddell CJ (1978) A new solid-phase sandwich radioimmunoassay and its application to the detection of snake venom. J Immunol Methods 23: 242–252Google Scholar
  30. Coulter AR, Harris RD, Sutherland SK (1980) Enzyme immunoassay for the rapid identification of snake venom. Med J Aust 1: 433–435PubMedGoogle Scholar
  31. Daudu I, Theakston RDG (1988) Preliminary trial of a new polyspecific antivenom in Nigeria. Ann Trop Med Parasitol 82: 311–313PubMedGoogle Scholar
  32. Detrait J (1982) Repertoire des instituts et laboratoires producteurs de serum antivenimeux. Bull Soc Herpet Fr 21: 44–56Google Scholar
  33. Editorial (1980) Antivenom therapy and reactions. Lancet i: 1009–1010Google Scholar
  34. Flowers HH (1963) Active immunisation of a human being against cobra (Naja naja) venom. Nature 200: 1017–1018PubMedGoogle Scholar
  35. Fraser TR (1895) The rendering of animals immune against the venom of the cobra and other serpents, and on the antidotal properties of the blood serum of the immunised animals. Br Med J 1: 1309–1312PubMedGoogle Scholar
  36. Freitas TV, Tavares AP, Theakston RDG, Laing G, New RRC (1988) Use of liposomes for protective immunisation against Crotalus durissus (tropical rattlesnake) venom. Toxicon 27: 341–347Google Scholar
  37. Ganthavorn S (1971) A case of king cobra bite. Toxicon 9: 293–294PubMedGoogle Scholar
  38. Grasset E, Christensen PA (1947) Enzyme purification of polyvalent antivenine, South and Equatorial African colubrine and viperine venoms. Trans R Soc Trop Med Hyg 41: 207–211PubMedGoogle Scholar
  39. Haast WE, Winer ML (1955) Complete and spontaneous recovery from the bite of a blue krait snake (Bungarus caeruleus). Am J Trop Med Hyg 4: 1135–1137PubMedGoogle Scholar
  40. Hickey AR, Wenger TL, Carpenter VP et al. (1991) Digoxin immune Fab therapy in the management of digitalis intoxication: Safety and efficacy results of an observational surveillance study. JACC 17: 590–598PubMedGoogle Scholar
  41. Ho M, Warrell MJ, Warrell DA, Bidwell D, Voller A (1986) A critical reappraisal of the use of enzyme-linked immunosorbent assays in the study of snake bite. Toxicon 24: 211–221PubMedGoogle Scholar
  42. Iddon D, Hommel M, Theakston RDG (1988) Characterization of a monoclonal antibody capable of neutralizing the haemorrhagic activity of West African Echis carinatus (carpet viper) venom. Toxicon 26: 167–179PubMedGoogle Scholar
  43. Iddon D, Theakston RDG, Hommel M (1985) A monoclonal antibody active against the “haemorrhagin” of Nigerian Echis carinatus venom. Toxicon 23: 576Google Scholar
  44. Iddon D, Theakston RDG, Ownby CL (1987) A study of the pathogenesis of local skin necrosis induced by Naja nigricollis (spitting cobra) venom using simple histological staining techniques. Toxicon 25: 665–672PubMedGoogle Scholar
  45. Johnson AG, Schmidtke J, Mreitt K, Han I (1968) Enhancement of antibody formation by nucleic acids and their derivatives. In: Plescia OJ, Braun W (eds) Nucleic acids in immunology. Springer-Verlag, Berlin, p 379Google Scholar
  46. Karlsson E, Arnberg H, Eaker D (1971) Isolation of the principal neurotoxins of two Naja naja subspecies. Eur J Biochem 21: 1–16PubMedGoogle Scholar
  47. Karlsson-Steiber C, Persson H (1994) Antivenom treatment in 30 cases of Vipera berus evenomation in Sweden 1985–1989. J Intern Med, in pressGoogle Scholar
  48. Kochalty WF, Bowles-Ledford E, Daly JG, Billings TA (1971) Preparation of coral snake antivenom from goat serum. Toxicon 9: 297–298Google Scholar
  49. Laing G, Theakston RDG, New RRC (1987) Use of liposomes incorporating immunostimulant for parenteral and oral immunization against snake venom. In: Gopalkrishnakone P, Tan CK (eds) Progress in venom and toxin research, Faculty of Medicine, University of Singapore, pp 283–295Google Scholar
  50. Laing G, Theakston RDG, New RRC (1988) Use of liposomes incorporating immunostimulants for immunisation against snake venom. Toxicon 26: 29Google Scholar
  51. Laing G, Theakston RDG, New RRC, Zumbuehl O, Parsley A (1986) Use of liposomes incorporating immunostimulants for immunization against snake venom. Trans R Soc Trop Med Hyg 80: 338Google Scholar
  52. Latin M (1978) Commercial production of commercial anti-snakebite serum (antivenin). In: Gans C, Gans KA (eds) Biology of the reptilia. Vol 8, Physiology. Academic Press, London, p 561Google Scholar
  53. Latifi M, Manhouri H (1966) Antivenin production. Mems Inst Butantan 33: 893–897Google Scholar
  54. Loprinzi CL, Hennessee J, Tamsky L et al. (1983) Snake antivenin administration in a patient allergic to horse serum. South Med J 76: 501–502PubMedGoogle Scholar
  55. Low BW, Corfield PWR (1988) The acetyl choline receptor: Identification of prime alpha-toxin binding site. In: Dolly JO (ed) Neurotoxins in neurochemistry. Ellis Horwood, Chichester, pp 13–26Google Scholar
  56. Luben RA, Mohler MA (1980) In vitro immunization as an adjunct to the production of hybridomas producing antibodies against the lymphokine osteoclast activating factor. Mol Immunol 17: 635–639PubMedGoogle Scholar
  57. Malasit P, Warrell DA, Chanthavanich P et al. (1986) Prediction, prevention and mechanism of early (anaphylactic) antivenom reactions in victims of snake bites. Br Med J 292: 17–20Google Scholar
  58. Meier J, Theakston RDG (1986) Approximate LD50 determinations of snake venoms using eight to ten experimental animals. Toxicon 24: 395–401PubMedGoogle Scholar
  59. Menez A (1985) Molecular immunology of snake toxins. Pharmacol Ther 30: 91–113PubMedGoogle Scholar
  60. Menez A, Boulain J-C, Grognet J-M et al. (1986) The use of monoclonal antibodies to elucidate the antigenic structure and the mechanisms of neutralization of snake toxins. In: Harris JB (ed) Natural toxins. Animal, plant and microbial. Clarendon Press, Oxford, pp 332–346Google Scholar
  61. Miller JFAP, Basten A, Sprent J, Cheers C (1971) Interaction between lymphocytes in immune responses. Cell Immunol 2: 469–495PubMedGoogle Scholar
  62. Minton SA (1974) Venom diseases. Charles C Thomas, Springfield, Illinois, p 171Google Scholar
  63. Mohamed AH, Bakr IA, Kamel A (1966) Egyptian polyvalent anti-snake-bite serum: Technique of preparation. Toxicon 4: 69–72PubMedGoogle Scholar
  64. New RRC, Theakston RDG, Zumbuehl O, Iddon D, Friend J (1984) Immunization against snake venoms. N Engl J Med 311: 56–57PubMedGoogle Scholar
  65. New RRC, Theakston RDG, Zumbuehl O, Iddon D, Friend J (1985) Liposomal immunization against snake venoms. Toxicon 23: 215–219PubMedGoogle Scholar
  66. Ownby CL, Odell GV, Woods GV, Colberg TR (1983) Ability of antiserum to myotoxin from a prairie rattlesnake (Crotalus viridis viridis) venom to neutralise local myotoxicity and lethal effects of myotoxin a and homologous crude venom. Toxicon 21: 35–41PubMedGoogle Scholar
  67. Perez JC, Garcia VE, Huang SY (1984) Production of a monoclonal antibody against the hemorrhagic activity of Crotalus atrox (western diamondback rattlesnake) venom. Toxicon 22: 967–973PubMedGoogle Scholar
  68. Phillips RE, Theakston RDG, Warrell DA et al. (1988) Paralysis, rhabdomyolysis and haemolysis caused by bites of Russell’s viper (Vipera russelli) in Sri Lanka: Failure of Indian (Haffkine) antivenom. Q J Med 68: 691–716PubMedGoogle Scholar
  69. Phisalix C, Bertrand G (1894) Sur la propriete antitoxique du sang des animaux vaccines contre le venin de vipere. C R Acad Sci 118: 356–358Google Scholar
  70. Pielak GJ, Mauk AG, Smith M (1985) Site-directed mutagenesis of cytochrome c shows that an invariant Phe is not essential for function. Nature 313: 152–154PubMedGoogle Scholar
  71. Pugh RNH, Theakston RDG (1987) Antivenom reactions and complement depletion in snake bite. Ann Trop Med Parasitol 81: 73–75PubMedGoogle Scholar
  72. Pukrittayakamee S, Esnouf MP, McMichael AJ (1983) Purification and inactivation of the factor X activator of Russell’s viper venom with monoclonal antibodies. Mol Biol Med 1: 123–135PubMedGoogle Scholar
  73. Reid HA (1964) Cobra bites. Br Med J 2: 540–544PubMedGoogle Scholar
  74. Reid HA (1968) Symptomatology, pathology and treatment of land snake bite in India and Southeast Asia. In: Bucherl W, Buckley EE, Deulofeu V (eds) Venomous animals and their venoms. Vol I, Venomous vertebrates. Academic Press, New York, pp 611–642Google Scholar
  75. Reid HA, Theakston RDG (1978) Changes in coagulation effects by venoms of Crotalus atrox as snakes age. Am J Trop Med Hyg 27: 1053–1057PubMedGoogle Scholar
  76. Rose ME, Orlans E (1962) Fowl antibody IV. The estimation of haemolytic fowl complement. Immunology 5: 642–648PubMedGoogle Scholar
  77. Rosenfeld G (1971) Symptomatology, pathology and treatment of snake bites in South America. In: Bucherl W, Buckley EE (eds) Venomous animals and their venoms. Vol II, Venomous vertebrates. Academic Press, New York, pp 345–384Google Scholar
  78. Russell FE (1961) Use of Crotalus monovalent antivenom from rabbit serum. C Ther Res 3: 438–440Google Scholar
  79. Russell FE (1980) Snake venom poisoning. Lippincott, Philadelphia, pp 797–810Google Scholar
  80. Russell FE (1988) Snake venom immunology: Historical and practical considerations. J Toxicol 7: 1–82Google Scholar
  81. Russell FE, Lauritzen L (1966) Antivenins. Trans R Soc Trop Med Hyg 60: 797–810PubMedGoogle Scholar
  82. Russell FE, Sullivan JB, Egan NB, Markland FS, Wingert WA, Bar-Or D (1985) Preparation of a new antivenom by affinity chromatography. Am J Trop Med Hyg 34: 141–150PubMedGoogle Scholar
  83. Russell FE, Timmerman WF, Meadows P (1970) Clinical use of antivenin prepared from goat serum. Toxicon 8: 63–65PubMedGoogle Scholar
  84. Sawai Y, Chinzei H, Kawamura Y, Fukuyama T, Okonogi T (1972) Studies on the improvement of treatment of Habu (Trimeresurus flavoviridis) bites. IX. Studies on the immunogenicity of the purified Habu venom toxoid by alcohol precipitation. Jpn J Exp Med 42: 155–164PubMedGoogle Scholar
  85. Schottler WHA (1951) Antigen-antibody relations in the present antivenin production of Brazil. Am J Trop Med 31: 500–509Google Scholar
  86. Sewall H (1887) Experiments on the preventive inoculation of rattlesnake venom. J Physiol 8: 203–210PubMedGoogle Scholar
  87. Smith DC, Reddi KR, Laing G, Theakston RDG, Landon J (1992) An affinity purified ovine antivenom for the treatment of Vipera berus envenoming. Toxicon 30: 865–871PubMedGoogle Scholar
  88. Smith TW, Lloyd BL, Spicer N, Haber E (1979) Immunogenicity and kinetics of distribution and elimination of sheep digoxin-specific IgG and Fab in the rabbit and baboon. Clin Exp Immunol 36: 384–396PubMedGoogle Scholar
  89. Sullivan JB (1987) Past, present and future immunotherapy of snake venom poisoning. Ann Emerg Med 16: 35–41Google Scholar
  90. Sullivan JB, Russell FE (1983) Isolation, quantitation and subclassing of IgG antibody to Crotalidae venom by affinity chromatography and protein electrophoresis. Toxicon suppl 3: 429–432Google Scholar
  91. Sutherland SK (1977) Serum reactions: An analysis of commercial antivenoms and the possible role of anticomplementary activity in de novo reactions to antivenoms and antitoxins. Med J Aust i: 613–615PubMedGoogle Scholar
  92. Taborska E (1971) Intraspecies variability of the venom of Echis carinatus. Physiol Bohemoslov 20: 307–318PubMedGoogle Scholar
  93. Tamiya T, Lamouroux A, Mien J-F et al. (1985) Cloning and sequence analysis of the cDNA encoding a snake neurotoxin precursor. Biochimie 67: 185–189PubMedGoogle Scholar
  94. Taylor D, Iddon D, Sells P, Semoff S, Theakston RDG (1986) An investigation of venom secretion by the venom gland cells of the carpet viper (Echis carinatus). Toxicon 24: 651–659PubMedGoogle Scholar
  95. Thalley BS, Carroll SB (1990) Rattlesnake and scorpion antivenoms from the egg yolks of immunized hens. Bio/Technology 8: 934–938PubMedGoogle Scholar
  96. Theakston RDG (1986) Characterization of venoms and standardization of antivenoms. In: Harris JB (ed) Natural toxins. Animal, plant and microbial. Clarendon Press, Oxford, pp 287–303Google Scholar
  97. Theakston RDG, Reid HA (1976) Effectiveness of Zagreb antivenom against envenoming by the adder, Vipera berus. Lancet i: 121–123Google Scholar
  98. Theakston RDG, Reid HA (1978) Changes in the biological properties of venom from Crotalus atrox with ageing. Periodicum Biologorum 80: 123–135Google Scholar
  99. Theakston RDG, Reid HA (1979) Enzyme-linked immunosorbent assay (ELISA) in assessing antivenom potency. Toxicon 17: 511–515PubMedGoogle Scholar
  100. Theakston RDG, Reid HA (1983) Development of simple standard assay procedures for the characterization of snake venoms. Bull WHO 61: 949–956PubMedGoogle Scholar
  101. Theakston RDG, Warrell DA (1991) Antivenoms: A list of hyperimmune sera currently available for the treatment of envenoming by bites and stings. Toxicon 29: 1419–1470PubMedGoogle Scholar
  102. Theakston RDG, Fan HW, Warrell DA et al. (1992) Use of enzyme immunoassays to compare the effect and assess dosage regimens of three Brazilian Bothrops antivenoms. Am J Trop Med Hygl, in pressGoogle Scholar
  103. Theakston RDG, Lloyd-Jones MJ, Reid HA (1977) Micro-ELISA for detecting and assaying snake venom and venom antibody. Lancet ii: 639–641Google Scholar
  104. Theakston RDG, Zumbuehl O, New RRC (1985) Use of liposomes for protective immunization in sheep against Echis carinatus snake venom. Toxicon 23: 921–925PubMedGoogle Scholar
  105. Virivan C, Veeravat U, Warrell MJ, Theakston RDG, Warrell DA (1986) ELISA confirmation of acute and past envenoming by the monocellate Thai cobra (Naja kaouthia). Am J Trop Med Hyg 35: 173–181Google Scholar
  106. Warrell DA, Davidson NMcD, Ormerod LD et al. (1974) Bites by the saw-scaled or carpet viper (echis carinatus): trial of two specific antivenoms. Br Med J 4: 437–440PubMedGoogle Scholar
  107. Warrell DA, Looareesuwan S, Theakston RDG et al. (1986) Randomised comparative trial of three monospecific antivenoms for bites by the Malayan pit viper (Calloselasma rhodostoma) in southern Thailand: Clinical and laboratory correlations. Am J Trop Med Hyg 35: 1235–1247PubMedGoogle Scholar
  108. Warrell DA, Warrell MJ, Edgar W et al. (1980) Comparison of Pasteur and Behringwerke antivenoms in envenoming by the carpet viper (Echis carinatus). Br Med J 1: 607–609Google Scholar
  109. Weber M, Changeux J-P (1974) Binding of Naja nigricollis [3H]α-toxin to membrane fragments from Electrophorus and Torpedo electric organs. II. Effect of cholinergic agonists and antagonists on the binding of the tritiated neurotoxin. Mol Pharmacol 10: 15–34PubMedGoogle Scholar
  110. Weir RC, Porter RR (1966) Comparison of the structure of the immunoglobulins from horse serum. Biochem J 100: 53–58Google Scholar
  111. WHO (1981) Progress in the characterization of venoms and standardization of antivenoms. Offset Publication No. 58. World Health Organisation, Geneva, p5Google Scholar
  112. Wiener S (1960) Active immunization of man against the venom of the Australian tiger snake (Notechis scutatus). Am J Trop Med Hyg 9: 284–292PubMedGoogle Scholar
  113. Wiener S (1961) Snake bite in a subject actively immunized against snake venom. Med J Aust 1: 658–659Google Scholar

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