The cultivation of HSV from infected secretions or tissues is the most widely used and most sensitive technique for the diagnosis of herpes simplex infection. Other techniques which may be used include the detection of viral particles or viral antigens in infected tissues, histopathological features consistent with HSV on biopsy, and the use of serological tests. The sensitivity of most of these techniques depends on the amount of virus in the specimen. This in turn is dependent on lesion stage (vesicles and ulcers contain more virus than crusts), whether the attack is primary or recurrent (more virus in the primary attack) and on the immune status of the patient (immunosuppressed patients generally have more virus).
KeywordsToxicity Fluorescein Thymidine Biotin Encephalitis
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