Abstract
The major problem in identifying the structural components of slow reacting substance of anaphylaxis (SRS-A) was the small quantities in which this mediator is released (Samuelsson 1983; Parker 1982). This also applies to the identification of the component leukotrienes in experimental systems. Certain identification of leukotrienes requires reverse phase HPLC, usually after an initial extraction procedure. Leukotrienes are recognised by their characteristic ultraviolet absorption (Matthews et al. 1981; Henke et al. 1984; Morris et al. 1978; Verhagen et al. 1984) in comparison with standards run in parallel or by structural confirmation of the HPLC products by gas chromatography and mass spectrometry. Such certainty requires large quantities of the initial product and allowance must be made for losses during extraction (Morris et al. 1983). The biological assay of SRS-A activity on guinea pig ileum is more sensitive but less specific and more difficult to quantify; nevertheless, it is arguable that it is biological activity rather than structure that is important. Radioimmunoassay (RIA) provides an alternative (Aeringhaus et al. 1982). The major problem with RIA is that class specific anti-sera for the individual leukotrienes are not yet available and cross-reactivity between the leukotrienes themselves and their isomers must always be borne in mind. The quantity of product which is recognised by a particular anti-serum as LTC4 is not necessarily the same as the quantity that would be identified by RP-HPLC followed by GC-MS.
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Flint, K.C. (1987). Newly Generated Mediators from Human Lung Mast Cells. In: Bronchoalveolar Mast Cells and Asthma. The Bloomsbury Series in Clinical Science. Springer, London. https://doi.org/10.1007/978-1-4471-1458-1_6
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