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MgO nanoparticles affect on the osteoblast cell function and adhesion strength of engineered tissue constructs

  • Morshed Khandaker
  • Kelli Duggan
  • Melissa Perram
Conference paper
Part of the Conference Proceedings of the Society for Experimental Mechanics Series book series (CPSEMS)

Abstract

The objective of this research was to evaluate the influence of magnesium oxide (MgO) on the adhesion strength between hard tissue and soft tissue constructs. The scope of works for this research were: (1) to determine the viability of osteoblast cells in hydrogel and hydrogel with 22 nm MgO particles, (2) to design and construct a test setup for the measurement of adhesion strength of engineered tissue constructs, and (3) to determine if MgO nanoparticles affect on the adhesion strength of the engineered tissue constructs. Mouse osteoblast cells (MT3T3E1) were cultured on polycaprolactone (PCL) scaffold, hydrogel scaffold, as well as hydrogel scaffold with 22 nm MgO particles. The viability of cells was determined in: hydrogel and hydrogel with 22 nm MgO particles. Tensile test were conducted on: (1) PCL-hydrogel, (2) PCL-hydrogel with cells, (3) PCL-hydrogel with cells and MgO nanoparticles, and (4) PCL-hydrogel with MgO nanoparticles to measure the adhesion strength between these hard tissue and soft tissue constructs. This research found the increase of osteoblast cells adhesion on hydrogel scaffold containing 22 nm MgO particles and decrease of adhesion strength between PCL and hydrogel, when both PCL and hydrogel were seeded with cells and 22 nm MgO particles.

Keywords

Osteoblast Cell Interface Strength Hydrogel Sample Tissue Construct Engineer Tissue Construct 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Morshed Khandaker
    • 1
  • Kelli Duggan
    • 1
  • Melissa Perram
    • 2
  1. 1.Department of Engineering and PhysicsUniversity of Central OklahomaEdmondUSA
  2. 2.Department of Chemical EngineeringPurdue UniversityWest LafayetteUSA

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