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Abstract

Amino acids in a given mixture are separated on the basis of differences in their solubilities and hence differential partitioning coefficients in a binary solvent system. The amino acids with higher solubilities in stationary phase move slowly as compared to those with higher solubilities in the mobile phase. The separated amino acids are detected by spraying the air dried chromatogram with ninhydrin reagent. All amino acids give purple or bluish purple colour on reaction with ninhydrin except proline and hydroxyproline, which give a yellow coloured product.

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Correspondence to Rajan Katoch .

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© 2011 Springer Science+Business Media, LLC

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Katoch, R. (2011). Chromatographic Separations. In: Analytical Techniques in Biochemistry and Molecular Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-9785-2_12

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