Chromatographic Separations

  • Rajan Katoch


Amino acids in a given mixture are separated on the basis of differences in their solubilities and hence differential partitioning coefficients in a binary solvent system. The amino acids with higher solubilities in stationary phase move slowly as compared to those with higher solubilities in the mobile phase. The separated amino acids are detected by spraying the air dried chromatogram with ninhydrin reagent. All amino acids give purple or bluish purple colour on reaction with ninhydrin except proline and hydroxyproline, which give a yellow coloured product.


Base Line Elution Volume Solvent Front Standard Amino Acid Ninhydrin Reagent 
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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Biochemistry Laboratory Department of Crop ImprovementCSKHPKVPalampurIndia

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