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Chromatographic Separations

  • Rajan Katoch
Chapter

Abstract

Amino acids in a given mixture are separated on the basis of differences in their solubilities and hence differential partitioning coefficients in a binary solvent system. The amino acids with higher solubilities in stationary phase move slowly as compared to those with higher solubilities in the mobile phase. The separated amino acids are detected by spraying the air dried chromatogram with ninhydrin reagent. All amino acids give purple or bluish purple colour on reaction with ninhydrin except proline and hydroxyproline, which give a yellow coloured product.

Keywords

Base Line Elution Volume Solvent Front Standard Amino Acid Ninhydrin Reagent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Biochemistry Laboratory Department of Crop ImprovementCSKHPKVPalampurIndia

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