Analysis of tissue-specific and PPARα-dependent induction of FABP gene expression in the mouse liver by an in vivo DNA electroporation method

  • Kensei Fujishiro
  • Yuka Fukui
  • Osamu Sato
  • Kohei Kawabe
  • Koichi Seto
  • Kiyoto Motojima
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 38)

Abstract

Peroxisome proliferator (PPAR)α ligand Wyl4,643 induces liver-fatty acid binding protein (FABP) spontaneously and heart-FABP gradually, but not intestine-FABP mRNA expression in the mouse liver. These strict regulations have not been reproduced in cultured cell systems. We applied a DNA electroporation method to directly introduce reporter gene constructs into the livers of mice. This system reproduced the in vivo responses of the above three FABP gene promoters to the PPARα ligand but not that of a promoter containing the typical three PPAR binding sites in tandem. Deletion and mutation analyses of the mouse L-FABP gene suggested that, in addition to the binding site for PPARα, a far upstream sequence is required for PPAR-dependent transactivation in the liver. In contrast to the cultured cell systems, our in vivo DNA electroporation method showed that PPARα binding to the promoter is necessary but not sufficient for PPARα ligand-dependent transcriptional activation of the L-FABP gene in vivo. (Mol Cell Biochem 239: 165–172, 2002)

Key words

fatty acid binding protein peroxisome proliferator-activated receptor transcription promoter 

Abbreviations

FABP

fatty acid binding protein

PPAR

peroxisome proliferator-activated receptor

PPRE

peroxisome proliferator-activated receptor responsive element

RXR

retinoid X receptor

Wy 14,643

4-chloro-6-(2,3-xylidino)-2-pyrimidinyl-thio)acetic acid

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Copyright information

© Springer Science+Business Media Dordrecht 2002

Authors and Affiliations

  • Kensei Fujishiro
    • 1
  • Yuka Fukui
    • 1
    • 2
  • Osamu Sato
    • 1
    • 2
  • Kohei Kawabe
    • 1
    • 2
  • Koichi Seto
    • 2
  • Kiyoto Motojima
    • 1
    • 2
    • 3
  1. 1.Department of Biochemistry, School of Pharmaceutical SciencesToho UniversityFunabashi, ChibaJapan
  2. 2.Department of BiochemistryMeiji Pharmaceutical UniversityKiyose, TokyoJapan
  3. 3.Department of BiochemistryMeiji Pharmaceutical UniversityTokyoJapan

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