Abstract
Colloidal gold techniques provide cellular markers (gold particles) that are spherical and extremely electron-dense (Fig. 151). One of the chief advantages of colloidal gold techniques is that the gold particles cannot be confused with any other cellular constituents, unlike the products of previously discussed ferritin and peroxidase/DAB/osmium staining procedures.
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References
Beesley, J.E. 1989. Colloidal gold: A new perspective for cytochemical marking. Royal Microscopical Society Microscopy Handbook #77. Oxford Science Publications, Oxford.
Bullock, G.R., and Petrusz, P. (eds.). 1982, 1983, 1985, 1989. Techniques in immunocytochemistry (Vols. 1–4). Academic Press, New York.
De Mey, J., and Moeremans, M. 1986. The preparation of colloidal gold probes and their use as marker in electron microscopy. In: J.K. Koehler (ed.), Advanced techniques in biological electron microscopy III, pp. 229–271. Springer-Verlag, New York.
Frens, G. 1973. Controlled nucleation for the regulation of the particle size in monodisperse gold Solutions. Nature (London) Phys. Sei. 241: 20.
Hayat, M.A. 1989 and 1991. Colloidal gold: Principles, methods and application. Academic Press, New York.
Horisberger, M. 1985. The gold method as applied to lectin cytochemistry in transmission and scanning electron microscopy. In: G.R. Bullock and P. Petrusz (eds.), Techniques in immunocytochemistry (Vol. 3, pp. 155–178). Academic Press, London.
Polak, J.M., and Varndell, I.M. 1984. Immunolabeling for electron microscopy. Elsevier, New York.
Slot, J., and Geuze, H. 1981. Sizing of protein A-colloidal gold probes for immunoelectron microscopy. J. Cell Biol. 90: 533.
References
DeMey, J. 1984. Colloidal gold as marker and tracer in light and electron microscopy. Bull. Electron. Microsc. Soc. Am. 14: 54.
Frens, G. 1973. Controlled nucleation for the regulation of the particle size in monodisperse gold Solutions. Nature Phys. Sei. 241: 20.
Hayat, M.A. 1989. Principles and techniques of electron microscopy. Biological applications, 3rd edn. CRC Press, Boca Raton, FL.
Reference
Hayat, M.A. 1989. Principles and techniques of electron microscopy. Biological applications, 3rd edn. CRC Press, Boca Raton, FL.
References
Bendayan, M., and Zollinger, M. 1983. Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold technique. J. Histochem. Cytochem. 31: 101.
Hayat, M.A. 1989. Principles and techniques of electron microscopy. Biological applications, 3rd edn. CRC Press, Boca Raton, FL.
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© 2003 Michael J. Dykstra
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Dykstra, M.J., Reuss, L.E. (2003). Techniques. In: Biological Electron Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-9244-4_12
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