Abstract
A good historical account of the beginnings of immunolabeling is found in a chapter by (1984). This history includes the work of (1941), who introduced fiuorescent- labeled antibodies to light microscopy, followed by the introduction of antibodies conjugated to ferritin (Singer, 1959), peroxidase (Nakane and Pierce, 1966), and gold (Faulk and Taylor, 1971; Romano et al., 1974). The ability to detect antibodies easily at both the light and electron microscopic level has made it possible to localize a variety of antigenic substances associated with the surfaces and interiors of cells. Modifications of these techniques have been used to demonstrate the presence of nucleic acids (Beesley, 2001; Raap et al., 1989; Silva et al., 1989), carbohydrates (Horisberger, 1985), and enzymes (Bendayan, 1985; Bendayan and Zollinger, 1983) as well. There are a number of excellent books dealing with immunolabeling at the light microscopic and ultrastructural level (Beesley, 1989a, Beesley, 1993; Bullock and Petrusz, 1982, 1983, 1985, 1989; Hayat, 1989, 1990; Polak and Van Noorden, 1983; Polak and Varndell, 1984; Shi et al., 2000; ternberger, 1986; Williams, 1977). Ritter and Ladyman (1995) assembled a book providing detailed techniques for producing monoclonal antibodies.
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Dykstra, M.J., Reuss, L.E. (2003). Immunocytochemistry. In: Biological Electron Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-9244-4_11
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