Arachidonic Acid Inhibits Cysteinyl-Leukotriene Receptor Activation in Human Pulmonary Vessels

  • Laurence Walch
  • Xavier Norel
  • Jean-Pierre Gascard
  • Charles Brink
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 525)

Abstract

There are a few reports suggesting that arachidonic acid may have direct regulatory actions in cells or tissues which are independent of the released endogenous metabolites. Brotherton and co-workers [1] reported that endothelial cells in culture which released prostacyclin in the presence of arachidonic acid became refractory subsequent to a second stimulation, that is, the cells failed to release significant levels of prostacyclin when compared with the initial challenge. These observations were also supported by the work of Revtyak and co-workers [2] who demonstrated that both histamine- and A23187-induced prostacyclin release was inhibited in human endothelial cells subsequent to arachidonic acid exposure. These results suggested that arachidonic acid may play a direct regulatory role via interaction with cell surface receptors. The aim of this investigation was to examine the effects of arachidonic acid on human pulmonary arteries and veins by measuring the release of several metabolites subsequent to the arachidonic acid exposure and evaluate the effects of cysteinyl-leukotrienes on this release.

Keywords

Tyrosine Histamine Prostaglandin Cytosol Thrombin 

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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Laurence Walch
    • 1
  • Xavier Norel
    • 1
  • Jean-Pierre Gascard
    • 2
  • Charles Brink
    • 1
  1. 1.2536 Hôpital BroussaisCNRS FREParisFrance
  2. 2.Centre Chirurgical Marie-LannelongueLe Plessis RobinsonFrance

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