Abstract
It is well known that when differentiated cells such as hepatocytes are cultured in vitro, they rapidly lose their specific features.
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References
Baudhuin, P., 1974, Isolation of rat liver peroxisomes. Method. Enzymol 31: 358–368.
Beken, S., Pauwels, M, Pahernik, S., Koebe, H.-G., Vercruysse, A., and Rogiers, V., 1997a, Collagen gel sandwich and immobilization cultures of rat hepatocytes: Problems encountered in expressing glutathione S-transferase activities. Toxic in Vitro 11: 741–752.
Beken, S., Tytgat, T., Pahernik, S., Koebe, H.-G., Vercruysse, A., and Rogiers, v., 1997b, Cell morphology, albumin secretion and glutathione S-transferase expression in collagen gel sandwich and immobilization cultures of rat hepatocytes. Toxic in Vitro 11: 409–416
Blaauboer, B.J., van Holsteijn, C.W., Bleumink, R., Mennes, W.C., van Pelt, F.N., Yap, S.H., van Pelt, J.F., van Iersel, A.A.J., Timmerman, A., and Schmid, B.P., 1990, The effect of beclobric acid and clofibric acid on peroxisomal beta-oxidation and peroxisome proliferation in primary cultures of rat, monkey and human hepatocytes. Biochem. Pharmacol 40: 521–528.
Bradford, M.A., 1976, Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Bochem. 72 : 248–254.
De Smet, K., Beken, S., Vanhaecke, T., Pauwels, M., Vercruysse, A., and Rogiers, V., 1998, Isolation of rat hepatocytes. In Methods in Molecular biology. Cytochrome P450 Protocols. Vol 107, (I.R. Phillips and E.A. Shephard, eds.) Humana Press, Totowa, pp. 295–301.
Depreter, M., Nardacci, R., Tytgat, T., Espeel, M., Stefanini, S., and Roels, F., 1998, Maturation of the liver specific peroxisome versus laminin I, collagen IV and integrin expression. Biol. Cell 90: 641–652.
Depreter, M., Tytgat, T., Beken, S., Espeel, M., De Smet, K., Rogiers, V., and Roels, F., 2000, Effects of extracellular matrix on the expression of peroxisomes in primary rat hepatocyte cultures. J. Hepatol 32: 381–391.
Depreter, M., Walker, T., De Smet, K., Beken, S., Kerckaert, I., Rogiers, V., and Roels, F., 2002, Hepatocyte polarity and the peroxisomal compartment: a comparative study, Histoch.J., 34: 139–151.
Dunn, J.C.Y., Yarmush, M.L., Koebe, H.-G. and Tompkins, R.G., 1989, Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration. FASEBJ., 3, 174–177.
Espeel, M., Mandel, H., Poggi, F., Smeitink, J.A.M., Wanders, R.J.A., Kerckaert, I., Schutgens, R.B.H., Saudubray, J.M., Poll-The, B.T., and Roels, F., 1995, Peroxisome mosaicism in the livers of peroxisomal deficiency patients. Hepatology 22: 497–504.
Flendrig, L.M., Te Velde A.A., Chamuleau, R.A., 1997, Semipermeable hollow fiber membranes in hepatocyte bioreactors: a prerequisite for a successful bioartificial liver ? Artif. Organs, 21: 1177–1181.
Kanai, M., Mabuchi, Y., Soji, T. and Herbert, D.C., 1996, Developmental changes of cell junctions and cell surface polarity in fetal rat hepatocytes: giving special consideration to Mg2+, Ca2+-ATPase activity. In Proceedings of the Xth International Congress on Histochemistry and Cytochemistry. (T. Saito ed.). Acta Histochem. Cytochem. 29 (Suppl 1), pp. 633–634.
Lazarow P.B., 1977, Three hypolipidemic drugs increase hepatic palmityl-coenzyme A oxidation in the rat. Science, 197: 580–581.
Lee, T.N., Araki, N. and Takashima, Y., 1993, Development of the bile canalicular system in rat liver: with special reference to bile canalicular actin filaments and Mg2+, Ca2+- ATPase activity. Acta Histochem. Cytochem. 26: 337–347.
Mitchell A.M., Bridges J.W., Elcombe C.R., 1984, Factors influencing peroxisome proliferation in cultured rat hepatocytes. Arch. Toxicol. 55: 239–246.
Patzer, J.F. (2001) Advances in Bioartificial Liver Assist. Devices. In: Bioartificial organs III: Tissue sourcing, immunoisolation, and clinical trials, (D. Hunkeler, A. Cherrington, A. Prokop, R. Rajotte, eds.) Annals NY Acad. Sci, Vol 944, Part IV.
Roels, F., De Prest, B., De Pestel, G., 1995, Liver and chorion cytochemistry. In Diagnosis of human peroxisomal disorders. A handbook. (F. Roels F, S. De Bie, R.B.H. Schutgens, and G.T.N. Besley, eds.). J. Inher. Metab. Dis. 18 Suppl 1: 155–171.
Sun, Y., Colburn, N.H., and Oberley, L.W. Depression of catalase gene expression after immortalization and transformation of mouse liver cells. Carcinogenesis, 14: 1505–1510.
Uusitalo, R.J. and Karnovsky, M.J., 1977, Surface localization of 5NTase on the mouse lymphocyte. J.Histochem.Cytochem. 2: 87–96.
Vanhaecke, T., De Smet, K., Beken, S., Pauwels, M., Vercruysse, A., and Rogiers, V., 1998, Primary cultures and co-cultures of rat hepatocytes. In Methods in Molecular biology. Cytochrome P450 Protocols. Vol 107,(I.R. Phillips and E.A. Shephard, eds.) Humana Press, Totowa, pp. 311–317.
Wachstein, M. and Meisel, E., 1957, Histochemistry of hepatic phosphatases at a physiologic pH. Am. J. Clin. Pathol. 27: 13–23.
Walker, T.M., Rhodes, P.C., and Westmoreland, C, 2000, The differential cytotoxicity of methotrexate in rat hepatocyte monolayer and spheroid cultures. Toxicol, in Vitro, 14: 475–485.
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Depreter, M. et al. (2004). Modified Peroxisomes in Primary Hepatocyte Cultures. In: Roels, F., Baes, M., De Bie, S. (eds) Peroxisomal Disorders and Regulation of Genes. Advances in Experimental Medicine and Biology, vol 544. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-9072-3_33
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DOI: https://doi.org/10.1007/978-1-4419-9072-3_33
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