Three-Dimensional Structural Analysis of Individual Myosin Heads Under Various Functional States

  • Eisaku Katayama
  • Norihiko Ichise
  • Naoki Yaeguchi
  • Tsuyoshi Yoshizawa
  • Shinsaku Maruta
  • Norio Baba
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 538)


Half a century has passed since the dedicated studies on the contraction mechanisms of muscle began, with considerable knowledge on its molecular architecture. Two major hypotheses were raised very early, one, ”sliding filament theory”,1,2 and the other, “crossbridge theory”. 3 The former was readily accepted, because the phenomenon was apparently visible under optical microscope. The latter, however, has been hindered from thorough experimental proof even now, though nothing other than crossbridges connect thick and thin filaments enabling force development. The original idea postulated the rowing movement of actin-bound myosin head coupled with ATP hydrolysis, but it was later replaced by swinging of the “lever-arm” moiety,4 according to the discovery of intramolecular bending by X-ray crystallography.5-8 One of the major reasons for such persistent difficulty to prove this simple hypothesis might be the lack of means to directly observe the actual structural change of working crossbridges with time and spatial resolution enough to visualize the fine details of the molecular nano-machine. Though the crystal structure of each component; actin9, 10 and myosin subfragment-1 (S1) with or without various nucleotides,5-8 was determined ten years ago, none of their complexed form was solved nor might be the subject matter for easy crystallization.


Actin Filament Myosin Head Essential Light Chain Myosin Motor Domain Scallop Myosin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Eisaku Katayama
    • 1
  • Norihiko Ichise
    • 1
  • Naoki Yaeguchi
    • 2
  • Tsuyoshi Yoshizawa
    • 3
  • Shinsaku Maruta
    • 3
  • Norio Baba
    • 2
  1. 1.Division of Biomolecular Imaging, Institute of Medical ScienceThe University of TokyoMinato-ku, TokyoJapan
  2. 2.Department of Electric EngineeringKogakuin UniversityHachioji, TokyoJapan
  3. 3.Department of Bioengineering, Faculty of EngineeringSoka UniversityHachioji, TokyoJapan

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