Abstract
Secretory granules were isolated from bovine hypothalamus and neurohypophysis, dissected out immediately after the animals were slaughtered. Homogenization of the tissues and the procedures of granule preparation, purification, and lysis were carried out in the medium containing protease inhibitors (phenylmethylsulphonyl fluoride, benzamidine, and leupeptin)31,45.
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© 2004 Springer Science+Business Media New York
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Galoyan, A.A. (2004). Neurosecretion of Cytokines by N. Para-Ventricularis and N. Supraopticus of Hypothalamus. In: Brain Neurosecretory Cytokines. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-8893-5_2
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DOI: https://doi.org/10.1007/978-1-4419-8893-5_2
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-4709-5
Online ISBN: 978-1-4419-8893-5
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