Abstract
The corneal pocket assay consists of the placement of a stimulus (purified growth factor, cell suspension, tumor tissue) into a corneal pocket in order to induce a vascular outgrowth from the limbal vessels toward the implant. This assay, with respect to the other in vivo assays, such as the chorio-allantoic membrane, has the advantage of measuring only new blood vessels, since the cornea is normally avascular. By using rabbits, additional advantages are gained like the easy manipulation of the animals and monitoring of the neovascular growth and inflammatory reaction over time. Measurement of corneal angiogenesis is useful to quantify the effects of angiogenic stimuli and to evaluate the efficacy of potential inhibitors of neovascularization following local delivery or systemic treatment.
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References
Gallo O., Masini E., Morbidelli L., et al. Role of nitric oxide in angiogenesis and tumor progression in head and neck cancer. J Natl Cancer Inst 1998; 90: 587–96.
Gimbrone M. Jr., Cotran R., Leapman S.B., Folkman J. Tumor growth and neovascularization: An experimental model using the rabbit cornea. J Natl Cancer lnst 1974; 52: 413–27.
Morbidelli L., Birkenhaeger R., Roeckl W., Granger H.J., Kaerst U., Weich H., Ziche M. Distinct capillary density and progression promoted by vascular endothelial growth factor-A homodimers and heterodimers. Angiogenesis, 1997; 1: 117–30.
Ziche M., Alessandri G., Gullino P.M. Gangliosides promote the angiogenic response. Lab. Invest. 1989; 61: 629–34.
Ziche M., Jones J., Gullino P.M. Role of prostaglandinEl and copper in angiogenesis. J Natl Cancer Inst 1982; 69: 475–82.
Ziche M., Morbidelli L., Choudhuri R., et al.. Nitric oxide synthase lies downstream from vascular endothelial growth factor-induced but not basic fibroblast growth factor-induced angiogenesis. J Clin Invest 1997a; 99: 2625–34.
Ziche M., Morbidelli L., Masini E., Amerini S., Granger H.J., Maggi C.A., Geppetti P., Ledda F. Nitric oxide mediates angiogenesis in vivo and endothelial cell growth and migration in vitro promoted by substance P. J Clin Invest 1994; 94: 2036–44.
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© 2004 Springer Science+Business Media New York
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Morbidelli, L., Ziche, M. (2004). The Rabbit Corneal Pocket Assay for the Study of Angiogenesis. In: Kirsch, M., Black, P.M. (eds) Angiogenesis in Brain Tumors. Cancer Treatment and Research, vol 117. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-8871-3_10
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DOI: https://doi.org/10.1007/978-1-4419-8871-3_10
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