A Molecular Tool Kit for Fungal Biotechnology
Fungal biotechnology increasingly relies on a set of tools to facilitate genetic manipulations for better economic outcomes (Table 3.1). In this chapter I will review some of the newer tools and technologies that can be used to advance biotechnology programs with fungi. For the purposes of this chapter I have focussed on tools applicable to heterotrophic filamentous ascomycetes, by far the most important fungi for biotechnology, but with some creativeness, these techniques can likely be adapted for a broader range of fungi. It is important to point out that the success or efficiency of any particular technology is generally fungal-specific. For example, rates of protoplast regeneration, transformation frequency, and degree of gene targeting are all variable depending on the fungal species and even the genetic background of particular strains.
KeywordsFilamentous Fungus Cryptococcus Neoformans Protoplast Fusion Aspergillus Nidulans Candida Glabrata
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