Slide Coating and DNA Immobilization Chemistries

  • Kathryn Aboytes
  • Jason Humphreys
  • Sonya Reis
  • Brian Ward

Abstract

Printing DNA microarrays on glass microscope slides originated with the Brown group in the mid 1990s (Schena et al., 1995). Since then, the use of microarrays in hybridization based assays such as measuring comparative gene expression levels, has escalated precipitously. The choice of using glass as a microarray substrate stems from its low fluorescence, low cost, high heat resistance, and rigidity. With few exceptions (Kumar et al., 2000), it has been necessary to coat the glass surface to facilitate DNA immobilization/spotting. Furthermore, before glass can be coated, it must be prepared and cleaned to accept that coating. In brief, a rigorous glass-cleaning step is followed by slide coating, which is in turn followed by DNA immobilization using an attachment chemistry that is matched with the slide coating. The purpose of this chapter is to review current approaches for preparing glass slides for coating, coating the slides and immobilizing appropriately modified nucleic acids onto coated glass surfaces. In the end, this chapter should enable the scientist to choose an appropriately matched set of slide and immobilization chemistries, thus clarifying the seemingly myriad array of immobilization strategies.

Keywords

Ozone Amide Pyrolysis Disulfide Biotin 

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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Kathryn Aboytes
    • 1
  • Jason Humphreys
    • 1
  • Sonya Reis
    • 1
  • Brian Ward
    • 1
  1. 1.Sigma-Aldrich Corp.St. LouisUSA

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