Poly (3’-deoxyADP-ribosyl)ation of Proteins in Liver Chromatin Isolated from Rats Fed with Hepatocarcinogens
We have recently found that 3’-deoxyNAD is a good substrate for poly(ADP-ribose)polymerase (PADPRP) (1,2). In fact, we observed that PADPRP makes small linear oligomers of 3’-deoxyADP-ribose with an average size of 4 ADP-ribose residues. The main advantage of this approach is that the highly branched and complex polymers of ADP-ribose synthesized with NAD (3) are not observed. Therefore, the electrophoretic identification of poly(ADPribosyl)ated-polypeptides following incubation of biological samples possessing PADPRP activity with [32P] 3’-deoxyNAD is facilitated.
KeywordsMouse Mammary Tumor Virus Hyperplastic Nodule High Mobility Group Protein Chromatin Fraction Protein Acceptor
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