Poly (3’-deoxyADP-ribosyl)ation of Proteins in Liver Chromatin Isolated from Rats Fed with Hepatocarcinogens

  • Rafael Alvarez-Gonzalez
  • Phyllis Panzeter
  • David P. Ringer
  • Hilda Mendoza-Alvarez
Conference paper


We have recently found that 3’-deoxyNAD is a good substrate for poly(ADP-ribose)polymerase (PADPRP) (1,2). In fact, we observed that PADPRP makes small linear oligomers of 3’-deoxyADP-ribose with an average size of 4 ADP-ribose residues. The main advantage of this approach is that the highly branched and complex polymers of ADP-ribose synthesized with NAD (3) are not observed. Therefore, the electrophoretic identification of poly(ADPribosyl)ated-polypeptides following incubation of biological samples possessing PADPRP activity with [32P] 3’-deoxyNAD is facilitated.


Mouse Mammary Tumor Virus Hyperplastic Nodule High Mobility Group Protein Chromatin Fraction Protein Acceptor 
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Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Rafael Alvarez-Gonzalez
    • 1
  • Phyllis Panzeter
    • 2
  • David P. Ringer
    • 2
  • Hilda Mendoza-Alvarez
    • 2
  1. 1.Department of Microbiology and Immunology, Texas College of Osteopathic MedicineThe University of North TexasTexasUSA
  2. 2.Biomedical DivisionThe Samuel Roberts Noble Foundation, Inc.ArdmoreUSA

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