Expression of poly (ADP-ribose) polymerase in differentiating HL-60 cells

  • Pierre Moreau
  • Guy Poirier
  • Scott Kaufmann
Conference paper


Poly (ADP-ribose) polymerase (PARP) is a zinc finger protein that is activated by binding to DNA single-or double-strand breaks (reviewed in Althaus et al., 1987). Studies with inhibitors have suggested that PARP plays a role in homologous and heterologous recombination as well as DNA repair, These processes are closely associated to cell differentiation and proliferation. To better understand the regulation of PARP activity during cellular differentiation, we have determined the PARP mRNA and protein levels during the granulocytic differentiation of HL-60 cells induced by DMSO.


Zinc Finger Protein Cell Cycle Distribution PARP Activity DMSO Treatment Granulocytic Differentiation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Althaus, F.R.; Richter, C. ADP-Ribosylation of proteins: Enzymology and Biological Significance. Berlin: Springer Verlag. 1987CrossRefGoogle Scholar
  2. Cherney, B.; McBride, O.W.; Chen, D.; Alkhatib, H.; Bhatia, K.; Hensley, P.; Smulson, M.E. Proc. Natl. Acad. Sci. U.S.A. 84: 8370–8374; 1987.PubMedCrossRefGoogle Scholar
  3. Ikai, K.; Ueda, K.; Fukushima, M.; Hayaishi, O. Poly (ADP-ribose) synthesis, a marker of granulocyte differentiation. Proc. Nat. Acad. Sci. U.S.A. 77:3682–3685; 1980CrossRefGoogle Scholar
  4. Ikai, K.; Ueda, K.; Hayaishi, O.; Immunohistochemistry of poly(ADP-ribose). In: Hayaishi, O.; Ueda, K., eds. ADP-ribosylation reactions: Biology and Medicine. New York: Academic Press; 1982:p.339–360.Google Scholar
  5. Kaufmann, S.H.; McLaughlin, S.M.; Kastan, M.B.; Liu, L.F.; Karp, J.E.; Burke, P.J. Topoisomerase II levels during granulocytic maturation in vitro and in vivo. Cancer Res. 51:3534–3543; 1991.PubMedGoogle Scholar
  6. Kurosaki, T.; Ushiro, H.; Mitsuuchi, Y.; Suzuki, S.; Matsuda, M.; Matsuda, Y.; Katanuma, N.; Kangawa, K.; Matsuo, H.; Hirose, T.; Inayama, S.; Shizuta, Y. J. of Biol. Chem. 262:15990–15997; 1987.Google Scholar
  7. Leduc, Y.; Lawrence, J.J.; DeMurcia, G.; Poirier, G.G. Cell cycle regulation of poly(ADP-ribose) synthetase in FR3T3 cells. Biochim. Biophys. Acta 968:275–282; 1988.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Pierre Moreau
  • Guy Poirier
  • Scott Kaufmann

There are no affiliations available

Personalised recommendations