The Expression of ICAM-1 and P-Selectin Are Increased Following Hemorrhage and Resuscitation in Mice

  • Andrea J. Cohen
  • Robert Shenkar
  • Dietmar Vestweber
  • Rubin Tuder
  • York E. Miller
  • Edward Abraham
Chapter
Part of the NATO ASI Series book series (NSSA, volume 297)

Abstract

Acute inflammatory lung injury is a common clinical occurrence following trauma and blood loss and is characterized by massive neutrophil infiltration into the lungs. The mechanisms responsible for the increased susceptibility to acute lung injury remain incompletely understood. P-selectin and ICAM-1, two types of adhesion molecules, have been shown to participate in several forms of neutrophil-dependent lung injury. In order to examine the mechanisms which contribute to trafficking of neutrophils in the lungs and development of pulmonary injury following blood loss, we investigated in-vivo mRNA and immunohistochemical expression of P-selectin and ICAM-1 following hemorrhage and resuscitation.

Keywords

Agarose Electrophoresis Histamine Luminal Paraformaldehyde 

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Copyright information

© Plenum Press, New York 1998

Authors and Affiliations

  • Andrea J. Cohen
    • 1
    • 2
    • 3
  • Robert Shenkar
    • 1
  • Dietmar Vestweber
    • 4
  • Rubin Tuder
    • 1
  • York E. Miller
    • 1
    • 2
  • Edward Abraham
    • 1
  1. 1.Division of Pulmonary Sciences and Critical Care Medicine and Department of PathologyUniversity of Colorado Health Sciences CenterFreiburg- ZahringerGermany
  2. 2.Denver Veterans Affairs Medical CenterFreiburg- ZahringerGermany
  3. 3.National Jewish Center for ResearchFreiburg- ZahringerGermany
  4. 4.Spemann LaboratoriesMax-Plank-Institut fur ImmunobiologieFreiburg- ZahringerGermany

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