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CK2α loci in the human genome: Structure and transcriptional activity

  • Ute Wirkner
  • Walter Pyerin
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 27)

Abstract

Two CK2α loci are present in the human genome. First, locus 20p13, that contains the CK2α coding gene. It spans around 70 kb, is composed of 13 exons and shows homology to the respective gene in the nematode Caenorhabditis elegans. The translation start site is located in the second exon, the stop codon in exon 13. Two transcription start sites were identified, the further 5′ located site defines position 1 of the gene, the second site is located at position 50, respectively. The promoter region shows characteristics of a so-called house keeping gene: A high GC content, lack of aTATA-box and presence of several GC-boxes. By reporter gene assays, the promoter region of the CK2α gene could be located between position −256 and 144 relative to the first transcription start site. In the 3′ noncoding region of the CK2α gene, six polyadenylation signals were identified. As indicated by Northern blot analysis and by comparison with expressed sequence tags from the EMBL databank, the most 3′ located, active polyadenylation signal seems to be the fourth defining the end of the CK2α gene. The second CK2α locus is at 11p15. It contains a processed pseudogene, which shows all typical features of a processed sequence, such as absence of introns, short poly-A tail and direct flanking repeats. Interestingly, it contains a complete open reading frame and has potential promoter elements in its 5′ region. Nevertheless, no promoter activity could be detected in reporter gene assays. (Mol Cell Biochem 191: 59–64, 1999)

Key words

protein kinase CK2 genes gene structure and organization gene promoter activation human 

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Copyright information

© Springer Science+Business Media Dordrecht 1999

Authors and Affiliations

  • Ute Wirkner
    • 1
  • Walter Pyerin
    • 1
  1. 1.Biochemische Zellphysiologie, Deutsches KrebsforschungszentrumHeidelbergGermany

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