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Glycan Arrays to Decipher the Specificity of Plant Lectins

Conference paper
First Online:
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 705)

Abstract

In recent years, evidence has been accumulating that protein–carbohydrate interactions play an important role in host–pathogen interaction(s), development, cell–cell communication, and cell signaling. To study the protein–carbohydrate recognition phenomena that take place within or at the surface of a cell, it is requisite to have the appropriate tools for dissecting this type of interaction. During the past decade, microarray technology has successfully been introduced into the field of glycobiology. These carbohydrate or glycan microarrays allow the rapid and comprehensive screening of carbohydrate-binding proteins for interaction with a large set of carbohydrate structures and characterization of their carbohydrate-binding properties.

Keywords

Carbohydrate-binding activity Glycan array N-glycan Specificity 
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Notes

Acknowledgments

The financial support of the Research Council of Ghent University and the Fund for Scientific Research-Flanders (FWO grants G.0201.04 and G.0022.08) is gratefully acknowledged. This work was supported in part by the Consortium for Functional Glycomics under National Institutes of Health/National Institute of General Medical Sciences Grant GM62116.

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© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Laboratory of Biochemistry and Glycobiology, Department of Molecular BiotechnologyGhent UniversityGentBelgium

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