Evaluation of Lithium Naphthalocyanine (LiNc) Microcrystals for Biological EPR Oximetry

  • Ramasamy P. Pandian
  • Simi M. Chacko
  • M. Lakshmi Kuppusamy
  • Brian K. Rivera
  • Periannan KuppusamyEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 701)


A new paramagnetic crystalline material, namely, lithium naphthalocyanine (LiNc), whose electron-paramagnetic-resonance (EPR) line width is highly sensitive to oxygen content, has been evaluated for use as oximetry probe in cells and tissues. Previously,we reported on the synthesis, structural framework,magnetic and oxygen-sensing properties of LiNc microcrystalline powder (Pandian et al, J. Mater. Chem. 19, 4138, 2009). The material exhibited a single, sharp EPR line that showed a highly linear response of its width to surrounding molecular oxygen (pO2) with a sensitivity of 31.2 mG/mmHg. In the present study, we evaluated the suitability of this material for in vivo oximetry in biological systems. We observed that the probe was stable in tissues for more than two months without any adverse effect on its oxygen-sensing properties. We further demonstrated that the probe can be prepared in sub-micron sizes for uptake by stem cells. Thus, the high oxygen sensitivity, biocompatibility, and long-term stability in tissues may be useful for high-resolution EPR oximetry.


Electron Paramagnetic Resonance Electron Paramagnetic Resonance Spectrum Spin Probe Electron Paramagnetic Resonance Line Electron Paramagnetic Resonance Measurement 
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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Ramasamy P. Pandian
    • 1
  • Simi M. Chacko
    • 1
  • M. Lakshmi Kuppusamy
    • 1
  • Brian K. Rivera
    • 1
  • Periannan Kuppusamy
    • 1
    Email author
  1. 1.Davis Heart and Lung Research Institute, Department of Internal MedicineThe Ohio State UniversityColumbusUSA

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