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Regional Brain Blood Flow in Mouse: Quantitative Measurement Using a Single-Pass Radio-Tracer Method and a Mathematical Algorithm

  • K. Xu
  • K. Radhakrishnan
  • A. Serhal
  • F. Allen
  • J. C. LaManna
  • M. A. PuchowiczEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 701)

Abstract

We1 have developed a reliable experimental method for measuring local regional cerebral blood flows in anesthetized mice. This method is an extension of the well-established single-pass dual-label indicator method for simultaneously measuring blood flow and glucose influx in rat brains. C57BL6J mice (n = 10) were anesthetized and regional blood flows (ml/min/g) were measured using the radio-tracer method. To test the sensitivity of this method we used a mathematical algorithm to predict the blood flows and compared the two sets of results.Measured regional blood flows between 0.7 and 1.7 ml/min/g were similar to those we have previously reported in the rat. The predicted blood flows using an assumed linearly increasing arterial tracer concentration-versus-time profile (that is, a ramp) were similar to the valuesmeasured in the physiological experiments (R2 0.99; slope 0.91). Thus,measurements of local regional cerebral blood flow in anesthetized mice using a single-pass radio-tracer method appear to be reliable.

Keywords

Tracer Concentration Cereb Blood Flow Mathematical Algorithm Anesthetize Mouse Euclid Avenue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • K. Xu
    • 1
  • K. Radhakrishnan
    • 4
  • A. Serhal
    • 2
  • F. Allen
  • J. C. LaManna
    • 2
  • M. A. Puchowicz
    • 3
    Email author
  1. 1.Department of NeurologyCase Western Reserve UniversityClevelandUSA
  2. 2.Department of Physiology and BiophysicsCase Western Reserve UniversityClevelandUSA
  3. 3.Department of NutritionCase Western Reserve UniversityClevelandUSA
  4. 4.Department of Pathology and Cancer CenterUniversity of New Mexico School of MedicineAlbuquerqueUSA

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