Advertisement

Characterizing Breast Cancer Mouse Xenografts with T1ρ-MRI

A Preliminary Study
  • Lin Z. Li
  • He N. Xu
  • Ravinder Reddy
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 701)

Abstract

Previously three imaging methods, dynamic contrast enhanced magnetic resonance imaging (DCE-MRI), T1ρ -MRI, and low temperatureNADH/Fp (reduced nicotinamide adenine dinucleotide/oxidized flavoprotein) fluorescence imaging (redox scanning)were reported to differentiate themouse xenografts of a lessmetastatic human melanoma cell line A375P and a more metastatic line C8161. The more metastaticmelanoma is characterized by less blood perfusion/permeability andmore oxidized mitochondrial redox state in the tumor core and lower T1ρ relaxation time averaged across the tumor section. These featuresmay be useful for identifying imaging biomarkers for cancer metastatic potential. Here, we have employed T1ρ - and T2-weightedMRI to image mouse xenografts of two human breast cancer lines (more metastatic MDA-MB-231 and less metastatic MDA-MB-468) on a vertical bore 9.4- T Varian MR system. The preliminary results indicated that the more metastatic MDA-MB-231 tumors had shorter Txρ relaxation constants on average than the less metastaticMDA-MB-468 tumors, and Txρ relaxationmight be a potential biomarker of breast tumor metastatic potential. Distinct ring-like structures were observed on Txρ -weightedMR images of the breast tumors, indicating tumor core and rim difference. This observation appears to be consistent with the tumor core-rim difference previously observed by DCE-MRI and redox scanning on aggressive melanoma xenografts.

Keywords

Mouse Xenograft Dynamic Contrast Enhance Magnetic Resonance Imaging Relaxation Constant Relaxation Time Constant Tumor Core 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Li LZ, Zhou R, Xu HN et al (2009) Quantitative magnetic resonance and optical imaging biomarkers ofmelanomametastatic potential. Proceedings of the NationalAcademy of Sciences of the USA 106:6608-6613CrossRefGoogle Scholar
  2. 2.
    Li LZ, Xu HN, Ranji M et al (2009) Mitochondrial redox imaging for cancer diagnostic and therapeutic studies. Journal of Innovative Optical Health Sciences 2:325-341CrossRefGoogle Scholar
  3. 3.
    Xu HN, Nioka S, Glickson JD et al (2010) Quantitative mitochondrial redox imaging of breast cancer metastatic potential. Journal of Biomedical Optics, 15:036010PubMedCrossRefGoogle Scholar
  4. 4.
    Sieuwerts AM, Klijn JG and Foekens JA (1997) Assessment of the invasive potential of human gynecological tumor cell lines with the in vitro Boyden chamber assay: influences of the ability of cells to migrate through the filter membrane. Clinical & Experimental Metastasis 15:53-62Google Scholar
  5. 5.
    Wheaton AJ et al (2004) Pulse sequence for multislice T1ρ -weightedMRI.Magnetic Resonance in Medicine 51:362-369Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Radiology, School of MedicineUniversity of PennsylvaniaPhiladelphiaUSA

Personalised recommendations