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Interfacial Reactions of Ozone with Lipids and Proteins in a Model Lung Surfactant System

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Book cover Multiscale and Multiphysics Computational Frameworks for Nano- and Bio-Systems

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Abstract

Oxidative stresses from irritants such as hydrogen peroxide and ozone (O3) can cause dysfunction of the pulmonary surfactant (PS) in the human lung, resulting in chronic diseases of the respiratory tract. For identification of structural changes of major components of PS due to the heterogeneous reaction with O3, field induced droplet ionization (FIDI) mass spectrometry is utilized to probe the surfactant layer system. FIDI is a soft ionization method in which ions are extracted from the surface of micro liter volume droplets. We report the structurally specific oxidative changes of \(\textrm{SP-B}_{1-25}\) (a shortened version of human surfactant protein B) and 1-palmitoyl-2-oleoyl-sn-phosphatidylglycerol (POPG) due to reaction with O3 at the air-liquid interface. We also present studies of the interfacial oxidation of \(\textrm{SP-B}_{1-25}\) in a non-ionizable 1-palmitoyl-2-oleoyl-sn-glycerol monolayer as a model lung surfactant system, where the competitive oxidation of the two components is observed. Our results indicate that the heterogeneous reaction at the interface is different from that in the bulk phase. For example, we observe the hydroxyhydroperoxide and the secondary ozonide as major products of the heterogeneous ozonolysis of POPG. These products are metastable and difficult to observe in the bulk-phase. In addition, compared to the nearly complete homogeneous oxidation of \(\textrm{SP-B}_{1-25}\), only a subset of the amino acids known to react with ozone is oxidized in the hydrophobic interfacial environment. Combining these experimental observations with the results of molecular dynamics simulations provides an improved understanding of the interfacial structure and chemistry of a model lung surfactant system when subject to oxidative stress.

In part with permission from Kim, H. I.; Kim, H; Shin, Y. S.; Beegle, L. W.; Jang, S. S.; Neidholdt, E. L.; Goddard, W. A.; Heath, J. R.; Kanik, I.; Beauchamp, J. L. J. Am. Chem. Soc. 2010, 132 (7), 2254–2263. Copyright 2010 American Chemical Society.

In part with permission from Kim, H. I.; Kim, H; Shin, Y. S.; Beegle, L. W.; Goddard, W. A.; Heath, J. R.; Kanik, I.; Beauchamp, J. L. J. Phys. Chem. B 2010, 114 (29), 9496–9503. Copyright 2010 American Chemical Society.

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Acknowledgements

The research described in this paper was carried out at the Beckman Institute and the Noyes Laboratory of Chemical Physics at the California Institute of Technology, the Computational NanoBioTechnology Laboratory at Georgia Institute of Technology and Jet Propulsion Laboratory under a contract with the National Aeronautics and Space Administration and funded through the Director’s Research and Development Fund. We appreciate the support provided by the Beckman Institute Mass Spectrometry Resource Center. Partial support was also provided by the National Science Foundation (NSF) under grant No. CHE-0416381 (JLB, PI) and the National Cancer Institute under grant No. 5U54 CA119347 (JRH, PI).

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Authors and Affiliations

  1. Center for Materials Simulations & Design, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea

    Hyungjun Kim

  2. Materials and Process Simulation Center, California Institute of Technology, Pasadena, USA

    Hyungjun Kim

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Kim, H. (2011). Interfacial Reactions of Ozone with Lipids and Proteins in a Model Lung Surfactant System. In: Multiscale and Multiphysics Computational Frameworks for Nano- and Bio-Systems. Springer Theses. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7601-7_7

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