Biological Applications of Stimulated Parametric Emission Microscopy and Stimulated Raman Scattering Microscopy

  • Shin’ichiro Kajiyama
  • Yasuyuki Ozeki
  • Kiichi Fukui
  • Kazuyoshi Itoh


Two types of newly developed nonlinear-optical microscopy are presented, namely, stimulated parametric emission (SPE) microscopy and stimulated Raman scattering (SRS) microscopy, together with their biological applications. These techniques allow high-resolution 3D visualization of unstained living cells. SPE microscopy is also applied to time-lapse observation of dividing cells as well as simultaneous observation of fluorescence emitted from labeled organelles and SPE signal.


Second Harmonic Generation Stimulate Raman Scattering Third Harmonic Generation Nonresonant Signal Stimulate Raman Scattering Signal 
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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Shin’ichiro Kajiyama
  • Yasuyuki Ozeki
  • Kiichi Fukui
  • Kazuyoshi Itoh
    • 1
  1. 1.Department of Material and Life Science, Graduate School of EngineeringOsaka UniversitySuitaJapan

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