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Biological Applications of Stimulated Parametric Emission Microscopy and Stimulated Raman Scattering Microscopy

  • Shin’ichiro Kajiyama
  • Yasuyuki Ozeki
  • Kiichi Fukui
  • Kazuyoshi Itoh
Chapter

Abstract

Two types of newly developed nonlinear-optical microscopy are presented, namely, stimulated parametric emission (SPE) microscopy and stimulated Raman scattering (SRS) microscopy, together with their biological applications. These techniques allow high-resolution 3D visualization of unstained living cells. SPE microscopy is also applied to time-lapse observation of dividing cells as well as simultaneous observation of fluorescence emitted from labeled organelles and SPE signal.

Keywords

Second Harmonic Generation Stimulate Raman Scattering Third Harmonic Generation Nonresonant Signal Stimulate Raman Scattering Signal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    M. Chalfie, Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher, “Green fluorescent protein as a marker for gene expression,” Science, vol. 263, no. 5148, pp. 802–805 (1994).Google Scholar
  2. 2.
    W. Denk, J. H. Strickler, W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science, vol. 248, no. 4951, pp. 73–76 (1991).Google Scholar
  3. 3.
    S. W. Hell, “Far-field optical nanoscopy,” Science, vol. 316, no. 5828, pp. 1153–1158 (2007).Google Scholar
  4. 4.
    D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science, vol. 254, no. 5035, pp. 1178–1181 (1991).Google Scholar
  5. 5.
    M. A. A. Neil, R. Juskaitis, and T. Wilson, “Method of obtaining optical sectioning by using structured light in a conventional microscope,” Opt. Lett., vol. 22, no. 24, pp. 1905–1907 (1997).Google Scholar
  6. 6.
    P. J. Campagnola, M.-D. Wei, A. Lewis, and L. M. Loew, “High-resolution nonlinear optical imaging of live cells by second harmonic generation,” Biophys. J., vol. 77, no. 6, pp. 3341–3349 (1999).Google Scholar
  7. 7.
    Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, “Nonlinear scanning laser microscopy by third harmonic generation,” Appl. Phys. Lett., vol. 70, no. 8, pp. 922–924 (1997).Google Scholar
  8. 8.
    A. Zumbusch, G. R. Holtom, and X. S. Xie, “Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering,” Phys. Rev. Lett., vol. 82, no. 20, pp. 4142–4145 (1999).Google Scholar
  9. 9.
    M. Hashimoto, T. Araki, and S. Kawata, “Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration,” Opt. Lett., vol. 25, no. 24, pp. 1768–1770 (2000).Google Scholar
  10. 10.
    K. Isobe, S. Kataoka, R. Murase, W. Watanabe, T. Higashi, S. Kawakami, S. Matsunaga, K. Fukui, and K. Itoh, “Stimulated parametric emission microscopy,” Opt. Express, vol. 14, no. 2, pp. 786–793 (2006).Google Scholar
  11. 11.
    N. Dudovich, D. Oron, and Y. Silberberg, “Single-pulse coherently controlled nonlinear Raman spectroscopy and microscopy,” Nature, vol. 418, no. 6897, pp. 512–514 (2002).Google Scholar
  12. 12.
    H. M. Dang, T. Kawasumi, G. Omura, T. Umano, S. Kajiyama, Y. Ozeki, K. Itoh, and K. Fukui, “Three-dimensional unstained live-cell imaging using stimulated parametric emission microscopy,” Jpn. J. Appl. Phys., vol. 48, p. 097003 (2009).ADSCrossRefGoogle Scholar
  13. 13.
    H. M. Dang, G. Omura, T. Umano, M. Yamagiwa, S. Kajiyama, Y. Ozeki, K. Itoh, and K. Fukui, “Label-free imaging by stimulated parametric emission microscopy reveals a difference in hemoglobin distribution between live and fixed erythrocytes,” J. Biomed. Opt., vol. 14, p. 040506 (2009).ADSCrossRefGoogle Scholar
  14. 14.
    M. Yamagiwa, Y. Ozeki, G. Omura, T. Suzuki, S. Kajiyama, K. Fukui, and K. Itoh, “Nonlinear phase imaging using two-beam interferometry in stimulated parametric emission microscopy,” Jpn. J. Appl. Phys., vol. 48, p. 062501 (2009).ADSCrossRefGoogle Scholar
  15. 15.
    C. L. Evans, E. O. Potma, M. Puoris’haag, D. Côte’, C. P. Lin, and X. S. Xie, “Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy,” Proc. Natl. Acad. Sci. U.S.A., vol. 102, no. 46, pp. 16807–16812 (2005).Google Scholar
  16. 16.
    A. Volkmer, J.-X. Cheng, and X. S. Xie, “Vibrational imaging with high sensitivity via epidetected coherent anti-Stokes Raman scattering microscopy,” Phys. Rev. Lett., vol. 87, no. 2, pp. 23901–23904 (2001).Google Scholar
  17. 17.
    J. -X. Cheng, L. D. Book, and X. S. Xie, “Polarization coherent anti-Stokes Raman scattering microscopy,” Opt. Lett. vol. 26, no. 17, pp. 1341–1343 (2001).Google Scholar
  18. 18.
    E. O. Potma, C. L. Evans, and X. S. Xie, “Heterodyne coherent anti-Stokes Raman scattering (CARS) imaging,” Opt. Lett., vol. 31, no. 2, pp. 241–243 (2006).Google Scholar
  19. 19.
    F. Ganikhanov, C. L. Evans, B. G. Saar, and X. S. Xie, “High-sensitivity vibrational imaging with frequency modulation coherent anti-Stokes Raman scattering (FM CARS) microscopy,” Opt. Lett., vol. 31, no. 12, pp. 1872–1874 (2006).Google Scholar
  20. 20.
    C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, “Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy,” Science, vol. 322, no. 5909, pp. 1857–1861 (2008).Google Scholar
  21. 21.
    Y. Ozeki, F. Dake, S. Kajiyama, K. Fukui, and K. Itoh, “Analysis and experimental assessment of the sensitivity of stimulated Raman scattering microscopy,” Opt. Express, vol. 17, no. 5, pp. 3651–3658 (2009).Google Scholar
  22. 22.
    P. Nandakumar, A. Kovalev, and A Volkmer, “Vibrational imaging based on stimulated Raman scattering microscopy,” New J. Phys., vol. 11, p. 033026 (2009).ADSCrossRefGoogle Scholar
  23. 23.
    B. F. Levine, C. V. Shank, and J. P. Heritage, “Surface vibrational spectroscopy using stimulated Raman scattering,” IEEE J. Quantum Electron, vol. QE-15, no. 12, pp. 1418–1432 (1979).Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Shin’ichiro Kajiyama
  • Yasuyuki Ozeki
  • Kiichi Fukui
  • Kazuyoshi Itoh
    • 1
  1. 1.Department of Material and Life Science, Graduate School of EngineeringOsaka UniversitySuitaJapan

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