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Coloring Soybeans with Anthocyanins?

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Book cover The Biological Activity of Phytochemicals

Part of the book series: Recent Advances in Phytochemistry ((RAPT,volume 41))

Abstract

The seed coats of black soybean (Glycine max (L.) Merr.) accumulate all anthocyanins required for the red (cyanidin-), blue (delphinidin-), purple (petunidin-), and orange (pelargonidin-3-O-glucoside) coloration of plant tissues. Metabolic engineering of anthocyanin biosynthesis in black soybean may potentially be used to generate distinct colors for the visible identification of transgenic seeds. Presently the causal agents of black coloration in soybean seed coats are speculative, and factors such as anthocyanic vacuolar inclusions (AVIs), co-pigmentation, and oxidation are likely involved in generating the black phenotype. This chapter is a perspective on anthocyanin biosynthesis in black soybean, the present understanding of black coloration in plant tissues, and potential strategies for engineering seed colors in light of substantial equivalence.

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Acknowledgments

The authors thank Dr. Ammar Saleem (University of Ottawa) for his assistance with HPLC, Dr. Shea Miller for performing the microscopy, and Drs. Malcolm Morrison and Elroy Cober (Agriculture and Agri-Food Canada) for providing seeds and for their helpful discussions. We would also like to thank the reviewers for their helpful suggestions. The research was supported by an NSERC Discovery Grant and AAFC project (RBPI 126) to BM.

Author information

Authors and Affiliations

  1. Bioproducts and Bioprocesses, Research Branch, Agriculture and Agri-Food Canada, Ottawa, ON, K1A 0C6, Canada

    Nikola Kovinich

  2. Department of Biology, Carleton University, Ottawa-Carleton Institute of Biology, Ottawa, ON, K1S 5B6, Canada

    Nikola Kovinich & Brian Miki

  3. Department of Biology, University of Ottawa, Ottawa, ON, Canada

    John T. Arnason

  4. Department of Biological Sciences, Brock University, St. Catharines, ON, L2S 3A1, Canada

    Vincenzo De Luca

Authors
  1. Nikola Kovinich
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  2. John T. Arnason
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  3. Vincenzo De Luca
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  4. Brian Miki
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Corresponding author

Correspondence to Nikola Kovinich .

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Editors and Affiliations

  1. , Institute of Biological Chemistry, Washington State University, P.O. Box 646340, Pullman, 99164-6340, USA

    David R. Gang

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Kovinich, N., Arnason, J.T., De Luca, V., Miki, B. (2011). Coloring Soybeans with Anthocyanins?. In: Gang, D. (eds) The Biological Activity of Phytochemicals. Recent Advances in Phytochemistry, vol 41. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7299-6_4

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