Fluorescence can be used to determine the concentration of a substance quantitatively (see Fig. 6.1). A more thorough introduction into the various fluorescent techniques is given in [219]. Even very specialised techniques, discussed below, can also be performed on microscopes [141, 142].


Green Fluorescent Protein Fluorescence Lifetime Fluorescence Polarisation Static Quenching Transition Dipole Moment 
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© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.BiochemistryRoss University School of MedicineRoseauDominica

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