• Engelbert Buxbaum


The simplest device to measure interaction of a sample with light is the absorbance spectrophotometer. Monochromatic light is passed through the sample (or, in some cases, reflected from it). If absorption occurs, the light intensity that arrives at a detector is lower than what would arrive in the absence of the sample.


Monochromatic Light Isosbestic Point High Optical Quality Absorbance Spectrophotometer Hyperchromic Effect 
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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.BiochemistryRoss University School of MedicineRoseauDominica

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