Biophysical Chemistry of Proteins pp 409-413 | Cite as

# Temperature Effects on Binding Equilibrium and Reaction Rate

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## Abstract

This type of investigation is quite important for the characterisation of an enzyme: Such experiments can be performed on a Forbes-bar (see Fig. 44.1), that allows measurements at different temperatures in parallel. The standard Gibbs free energy of ligand binding

$$\Delta {G'}^{0} = -R {_\ast} T {_\ast}\ln (1/{K}_{\mathrm{ d}})\qquad \mathrm{at}\,\,25\mathrm{\!\circ \mathrm{C}}$$

(44.1)

$$\ln ({K}_{\mathrm{d}}) = -\dfrac{\Delta {H'}^{0}} {R} {_\ast} \dfrac{1} {T} + A$$

(44.2)

$$\Delta {S'}^{0} = \dfrac{\Delta {H'}^{0} - \Delta {G'}^{0}} {T}$$

(44.3)

^{1}Δ G*′*^{0}can be determined from the dissociation constant at standard temperature (25∘C)## Keywords

Activation Energy Differential Scanning Calorimetry Isothermal Titration Calorimetry Standard Temperature Photoacoustic Signal
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

## Copyright information

© Springer Science+Business Media, LLC 2011