General Conditions for Interpretable Results

  • Engelbert Buxbaum


In principle, any technique that separates bound from unbound ligand, or occupied from unoccupied protein, can be used to monitor binding. As a general rule, highest accuracy is achieved when the concentration of the protein in binding studies is approximately equal to K d (within about one order of magnitude either way), this makes it easier to determine the concentration difference between bound and total ligand. Since the supply of protein is usually a limiting factor in research, binding studies are expensive and the sample size must be reduced as much as possible. Considerable progress has been made in this direction over the years.


Binding Study Concentration Difference Considerable Progress Ligand Concentration Enzyme Kinetic 
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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.BiochemistryRoss University School of MedicineRoseauDominica

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