Isotope Exchange

  • Engelbert Buxbaum


Some reactions proceed via an intermediate that is stable enough to be detected with sensitive isotope techniques.One example is the transfer of the γ -phosphate group to aspartic acid in P-type ATPases, forming an acyl-phosphate intermediate, from which ADP is released followed by dephosphorylation:


Mass Spectrometry Reaction Mechanism Aspartic Acid Chromatographic Separation Phosphate Group 
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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.BiochemistryRoss University School of MedicineRoseauDominica

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