Abstract
An important step in the characterisation of a new protein is the determination of its primary structure (amino acid sequence). This can be determined either by repeated chemical cleavage of terminal amino acids (either from the N- or C-terminal end, see Fig. 30.1 and Fig. 30.2 respectively), or by MS/MS.
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In a superheterodyne receiver (“superhet”) the signal of frequency f s is mixed with a reference signal of frequency f r. The resulting beat signal of frequency f s − f r contains all the information of the original, but at a much lower, and therefore easier to handle, frequency.
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Buxbaum, E. (2011). Protein Sequencing. In: Biophysical Chemistry of Proteins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-7251-4_30
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DOI: https://doi.org/10.1007/978-1-4419-7251-4_30
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