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Chromatography

  • Engelbert Buxbaum
Chapter

Chromatographic Methods

In chromatography, analytes are bound to a solid support and then specifically eluted (see Fig. 14.1). Depending on the type of support we distinguish:
Paper chromatography

is the oldest method, the sample is spotted onto a paper strip, which is hung with its lower end into a solvent. Capillary action sucks the solvent upward past the sample. Separation usually occurs by partitioning of sample molecules between paper-bound water and the mobile organic phase.

Thin layer chromatography

uses a solid support (glass plates, plastic or aluminium sheets) covered with a thin (0. 1–2mm) layer of the separation matrix. This layer is prepared by grinding the matrix material to a fine powder, mixing it with water to a thick slurry, and pouring it over the plates. Because different materials can be used as a matrix, this method is more versatile than paper chromatography.

Column chromatography

uses columns filled with the matrix instead of thin sheets. The solvent is pushed...

Keywords

Ammonium Sulphate High Flow Rate Back Pressure Theoretical Plate Partition Ratio 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.BiochemistryRoss University School of MedicineRoseauDominica

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