Isolation of Organelles

  • Engelbert Buxbaum


After homogenisation the various cell organelles need to be separated from each other. This is done by fractionated centrifugation [103]. First, connective tissue, undamaged cells, and other debris are removed by a brief spin at low speed (10min at 500\(\mathfrak{g}\)). In the next step nucle and plasma membranes are spun down (10min at 3 000 \(\mathfrak{g}\)). Mitochondria, plastids, and heavy microsomes require about 30min at 20000\(\mathfrak{g}\) to be spun down, 1h at 100000\(\mathfrak{g}\) is required for light microsomes. The remaining supernatant contains the cytosolic proteins.


Plasma Membrane Molecular Mass Connective Tissue Purification Step Cytosolic Protein 
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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.BiochemistryRoss University School of MedicineRoseauDominica

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