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Isolation of Organelles

  • Engelbert Buxbaum
Chapter

Abstract

After homogenisation the various cell organelles need to be separated from each other. This is done by fractionated centrifugation [103]. First, connective tissue, undamaged cells, and other debris are removed by a brief spin at low speed (10min at 500\(\mathfrak{g}\)). In the next step nucle and plasma membranes are spun down (10min at 3 000 \(\mathfrak{g}\)). Mitochondria, plastids, and heavy microsomes require about 30min at 20000\(\mathfrak{g}\) to be spun down, 1h at 100000\(\mathfrak{g}\) is required for light microsomes. The remaining supernatant contains the cytosolic proteins.

Keywords

Plasma Membrane Molecular Mass Connective Tissue Purification Step Cytosolic Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.BiochemistryRoss University School of MedicineRoseauDominica

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