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Comparative Proteomic Analysis as a Method to Investigate Inflammatory and Neuropathic Pain

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Genomics, Proteomics, and the Nervous System

Part of the book series: Advances in Neurobiology ((NEUROBIOL,volume 2))

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Abstract

Patients suffering from pathological pain due to neuropathy or chronic inflammation are dependent on treatment with efficient and highly specific drugs. Although a number of analgesics are available many patients cannot be adequately treated because of lacking efficacy or severe side effects. The pain hypersensitivity in inflammatory and neuropathic pain is associated with changes of protein expression in the CNS which are not completely clarified at the moment. To develop novel treatment strategies it is necessary to elucidate the mechanisms of both pain states, and to find proteins that are specifically regulated in either neuropathic or inflammatory pain and that may become drug targets.

Proteomics by 2D-gel electrophoresis combined with MALDI-TOF mass spectrometry might help identifying regulated proteins in the nervous system in inflammatory or neuropathic pain models and therefore facilitate the development of novel analgesics. In these chapter a number of proteomic approaches in the field of inflammatory pain and nerve injury are reviewed which might provide starting points for further research in this field.

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Abbreviations

CCI:

chronic constriction injury

IEF:

isoelectric focusing

IPG:

immobilized pH gradient

MALDI-TOF MS:

matrix-assisted laser desorption ionization time-of-flight mass spectrometry

MW:

molecular weight

NC:

nerve crush

SNI:

spared nerve injury

SNL:

spinal nerve ligation

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  1. Pharmazentrum frankfurt/ZAFES, Institut für Klinische Pharmakologie, Klinikum der Johann Wolfgang Goethe-Universität Frankfurt, Theodor Stern Kai 7, 60590, Frankfurt am Main, Germany

    Ellen Niederberger

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  1. Ellen Niederberger
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  1. Nathan S. Kline Institute for, Psychiatric Research, New York University, Old Orangeburg Road 140, Orangeburg, 10962, New York, USA

    James D Clelland

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Niederberger, E. (2011). Comparative Proteomic Analysis as a Method to Investigate Inflammatory and Neuropathic Pain. In: Clelland, J. (eds) Genomics, Proteomics, and the Nervous System. Advances in Neurobiology, vol 2. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7197-5_22

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