Overview of Ancillary Methods in Lymph Node FNA diagnosis

Part of the Essentials in Cytopathology book series (EICP, volume 10)


Ancillary tests are critically important tools in the assessment of lymph node aspirates, as they can help establish the etiology of infectious lymphadenopathies, determine the type and primary site of metastatic malignancies, diagnose and subclassify lymphomas, and exclude malignancy in reactive lymphadenopathies. In addition, ancillary tests can also be used to provide prognostic information in certain neoplasms and help predict treatment response.


Follicular Lymphoma Hodgkin Lymphoma Mantle Cell Lymphoma Merkel Cell Carcinoma Burkitt Lymphoma 
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Suggested Reading

  1. Allen TC, Cagle PT (2009) Basic concepts of molecular pathology. Springer, New YorkCrossRefGoogle Scholar
  2. Angelakis E, Roux V, Raoult D, Rolain JM (2009) Real-time PCR strategy and detection of bacterial agents of lymphadenitis. Eur J Clin Microbiol Infect Dis 28(11):1363–1368PubMedCrossRefGoogle Scholar
  3. Bahrami A, Truong LD, Ro JY (2008) Undifferentiated tumor: true identity by immunohistochemistry. Arch Pathol Lab Med 132(3):326–348PubMedGoogle Scholar
  4. Caraway NP (2005) Strategies to diagnose lymphoproliferative disorders by fine-needle aspiration by using ancillary studies. Cancer 105(6):432–442PubMedCrossRefGoogle Scholar
  5. Cheng L, Zhang DY (2008) Molecular genetic pathology. Humana, TotowaCrossRefGoogle Scholar
  6. Craig FE, Foon KA (2008) Flow cytometric immunophenotyping for hematologic neoplasms. Blood 111(8):3941–3967PubMedCrossRefGoogle Scholar
  7. Dey P (2006) Role of ancillary techniques in diagnosing and subclassifying non-Hodgkin’s lymphomas on fine needle aspiration cytology. Cytopathology 17(5):275–287PubMedCrossRefGoogle Scholar
  8. Ellis DW, Eaton M, Fox RM, Juneja S, Leong AS, Miliauskas J, Norris DL, Spagnolo D, Turner J (2005) Diagnostic pathology of lymphoproliferative disorders. Pathology 37(6):434–456PubMedCrossRefGoogle Scholar
  9. Eyzaguirre E, Haque AK (2008) Application of immunohistochemistry to infections. Arch Pathol Lab Med 132(3):424–431PubMedGoogle Scholar
  10. Finn WG, Peterson LC (2004) Hematopathology in oncology. Kluwer, BostonCrossRefGoogle Scholar
  11. Fowler LJ, Lachar WA (2008) Application of immunohistochemistry to cytology. Arch Pathol Lab Med 132(3):373–383PubMedGoogle Scholar
  12. Garcia CF, Swerdlow SH (2009) Best practices in contemporary diagnostic immunohistochemistry: panel approach to hematolymphoid proliferations. Arch Pathol Lab Med 133(5):756–765PubMedGoogle Scholar
  13. Gudgin EJ, Erber WN (2005) Immunophenotyping of lymphoproliferative disorders: state of the art. Pathology 37(6):457–478PubMedCrossRefGoogle Scholar
  14. Higgins RA, Blankenship JE, Kinney MC (2008) Application of immunohistochemistry in the diagnosis of non-Hodgkin and Hodgkin lymphoma. Arch Pathol Lab Med 132(3):441–461PubMedGoogle Scholar
  15. Ioachim HL, Medeiros LJ (2009) Ioachim’s lymph node pathology. Wolters Kluwer Health/Lippincott Williams & Wilkins, PhiladelphiaGoogle Scholar
  16. Jevremovic D, Viswanatha DS (2009) Molecular diagnosis of hematopoietic and lymphoid neoplasms. Hematol Oncol Clin North Am 23(4):903–933PubMedCrossRefGoogle Scholar
  17. Jones D (2010) Neoplastic hematopathology: experimental and clinical approaches. Humana, TotowaCrossRefGoogle Scholar
  18. Jorgensen JL (2005) State of the Art Symposium: flow cytometry in the diagnosis of lymphoproliferative disorders by fine-needle aspiration. Cancer 105(6):443–451PubMedCrossRefGoogle Scholar
  19. Nguyen DT, Diamond LW, Braylan RC (2007) Flow cytometry in hematopathology a visual approach to data analysis and interpretation. Humana, TotowaGoogle Scholar
  20. O’Leary TJ, Abbondanzo SL, Frisman DM (2003) Advanced diagnostic methods in pathology: principles, practice, and protocols. W.B. Saunders, PhiladelphiaGoogle Scholar
  21. Pfeifer JD, Arber DA (2006) Molecular genetic testing in surgical pathology. Lippincott Williams & Wilkins, PhiladelphiaGoogle Scholar
  22. Spagnolo DV, Ellis DW, Juneja S, Leong AS, Miliauskas J, Norris DL, Turner J (2004) The role of molecular studies in lymphoma diagnosis: a review. Pathology 36(1):19–44PubMedCrossRefGoogle Scholar
  23. Taylor CR (2009) IHC and the WHO classification of lymphomas: cost effective immunohistochemistry using a deductive reasoning “decision tree” approach. Appl Immunohistochem Mol Morphol 17(5):366–374PubMedCrossRefGoogle Scholar
  24. Taylor CR (2009) The WHO classification of lymphomas: cost-effective immunohistochemistry using a deductive reasoning “decision tree” approach: part II: the decision tree approach: diffuse patterns of proliferation in lymph nodes. Appl Immunohistochem Mol Morphol 17(6):470–482PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Lab. Medicine & PathologyUniversity of MinnesotaMinneapolisUSA
  2. 2.Outpatient Pathology AssociatesSacramentoUSA

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