Identification and Validation of ISG15 Target Proteins

  • Larissa A. Durfee
  • Jon M. Huibregtse
Part of the Subcellular Biochemistry book series (SCBI, volume 54)


ISG15 is an interferon-induced ubiquitin-like protein (Ubl) that has antiviral properties. The core E1, E2 and E3 enzymes for conjugation of human ISG15 are Ube1L, UbcH8 and Herc5, all of which are induced at the transcriptional level by Type 1 interferon signaling. Several proteomics studies have, together, identified over 300 cellular proteins as ISG15 targets. These targets include a broad range of constitutively expressed proteins and approximately 15 interferon-induced proteins. This chapter provides an overview of the target identification process and the validation of these targets. We also discuss the limited number of examples where the biochemical effect of ISG15 conjugation on target proteins has been characterized.


Human Physiology Identification Process Target Protein Cellular Protein Transcriptional Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Landes Bioscience and Springer Science+Business Media 2010

Authors and Affiliations

  1. 1.Section of Molecular Genetics and Microbiology, Institute for Cellular and Molecular BiologyThe University of Texas at AustinAustinUSA

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