Optical Mapping and Calcium Imaging
This chapter will cover basic theory, principles, and applications of optical mapping of transmembrane potentials, as well as of intracellular calcium imaging. Calcium plays a central role in both electrical and mechanical function of excitable tissue, and serves as an important player in many biochemical and biophysical cellular regulatory functions. Emphasis will be placed on practical applications, advantages, and limitations of both techniques.
KeywordsExcitation Light Light Guide Optical Mapping Tissue Depth Excitation Light Intensity
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