Optical Mapping and Calcium Imaging



This chapter will cover basic theory, principles, and applications of optical mapping of transmembrane potentials, as well as of intracellular calcium imaging. Calcium plays a central role in both electrical and mechanical function of excitable tissue, and serves as an important player in many biochemical and biophysical cellular regulatory functions. Emphasis will be placed on practical applications, advantages, and limitations of both techniques.


Excitation Light Light Guide Optical Mapping Tissue Depth Excitation Light Intensity 


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© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of Biomedical Engineering, College of EngineeringUniversity of Alabama at BirminghamBirminghamUSA

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