High Endothelial Venule Reporter Mice to Probe Regulation of Lymph Node Vasculature

  • Kevin L. Bentley
  • Sharon Stranford
  • Shan Liao
  • Rawad M. Mounzer
  • Frank H. Ruddle
  • Nancy H. Ruddle
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 691)


Lymphotoxin (LT) is crucial for the regulation of HEV adhesion molecules MAdCAM-1 and PNAd and a sulfotransferase, GlcNAc6ST-2 (gene symbol Chst4); here called HEC-6ST. Following immunization, some HEVs express markers of both HEVs (PNAd) and LVs (LYVE-1). In order to evaluate this process in real time, we have developed mice transgenic for a construct that consists of an HEV specific gene driving a green fluorescent reporter gene (eGFP). These mice express the reporter gene in HEVs in concurrence with the endogenous gene and PNAd. Additional mice transgenic for lymphatic vessel reporter constructs are in development. These will provide material for in vivo imaging and allow us to evaluate the regulation and interaction of HEVs and LVs.


Green Fluorescent Protein Reporter Mouse Afferent Lymphatic Vessel SV40 Polyadenylation Signal Mucosal Addressin Cell Adhesion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



lymph node


peripheral lymph node


lymphotoxin-β receptor


lymphatic vessel


high endothelial venule


peripheral node addressin


high endothelial cell sulfotransferase, HEC-GlcNA6ST, GlcNAc6ST-2, N-acetyl glucosamine 6-O-sulfotransferase-2; carbohydrate (chondroitin 6/keratan) sulfotransferase 4


mucosal addressin cell adhesion molecule 1


glycosylation-dependent cell adhesion molecule-1




lymphatic vessel endothelial hyaluronan receptor 1


green fluorescent protein




prospero-related homeobox 1



We thank Myriam Hill for figure preparation and Myriam Hill and Daniel Reagan for outstanding technical assistance. This work was supported by U.S. Public Health Service Grants R01 DK057731 and CA016885 (to N.H.R.) and GM09966 (to F.H.R.) from the National Institutes of Health, a Mount Holyoke College Faculty Research Fellowship (to S.S) and a Lymphatic Research Foundation Fellowship (to R.A.M.)


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Kevin L. Bentley
    • 1
  • Sharon Stranford
    • 2
  • Shan Liao
    • 1
    • 3
  • Rawad M. Mounzer
    • 1
    • 4
  • Frank H. Ruddle
    • 5
  • Nancy H. Ruddle
    • 1
    • 6
  1. 1.Department of Epidemiology and Public HealthYale University School of MedicineNew HavenUSA
  2. 2.Department of Biological SciencesMount Holyoke CollegeSouth HadleyUSA
  3. 3.Department of Radiation OncologyE.L. Steele Laboratory for Tumor Biology, Massachusetts General HospitalBostonUSA
  4. 4.Department of Internal MedicineUPMC Montefiore HospitalPittsburghUSA
  5. 5.Department of Molecular, Cellular, and Developmental BiologyYale UniversityNew HavenUSA
  6. 6.Departments of Epidemiology and Public Health and ImmunobiologyYale University School of MedicineNew HavenUSA

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