Understanding Life and Death at CD95

  • Carina Pforr
  • Leo Neumann
  • Roland Eils
  • Peter H. Krammer
  • Inna N. Lavrik
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 691)


CD95 (APO-1/Fas) is a member of the death receptor (DR) family, a subfamily of the TNF-R (tumor necrosis factor receptor) superfamily. Engagement of CD95 leads to the induction of the apoptotic and non-apoptotic signaling pathways. Here we addressed the question of crosstalk between CD95-induced apoptosis and NF-κB activation. Using HeLa-CD95 cells (HeLa cells stably overexpressing CD95) we have demonstrated that CD95 stimulation results in the induction of both life and death pathways. We have shown that DED proteins of the DISC, procaspase-8 and c-FLIP, regulate both pathways in a dynamic way. The data generated in this study are currently used for building a systems biology model of life/death decisions at CD95.


CD95 Stimulation Effector Caspase Activity Total Cellular Lysate CD95 Disc Death Receptor Stimulation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We acknowledge support by the Helmholtz Alliance on Systems Biology (NW1SBCancer/UniHD) and the BMBF funded ForSys centre VIROQUANT (0313923). We also thank the Wilhelm Sander Stiftung, Helmholtz-Russia Joint Research Groups-2008-2, SFB 405 and the Tumorzentrum Heidelberg/Mannheim for supporting our work.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Carina Pforr
    • 1
  • Leo Neumann
    • 2
  • Roland Eils
    • 3
    • 4
    • 5
  • Peter H. Krammer
    • 1
  • Inna N. Lavrik
    • 1
    • 4
  1. 1.Division of ImmunogeneticsGerman Cancer Research Center (DKFZ)HeidelbergGermany
  2. 2.Division of Theoretical BioinformaticsGerman Cancer Research Center (DKFZ)HeidelbergGermany
  3. 3.Division of Theoretical BioinformaticsGerman Cancer Research Center (DKFZ)HeidelbergGermany
  4. 4.BioquantHeidelbergGermany
  5. 5.Department for Bioinformatics and Functional GenomicsInstitute for Pharmacy and Molecular Biotechnology, University of HeidelbergHeidelbergGermany

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