Monitoring Population Membrane Potential Signals from Neocortex
Voltage-sensitive dyes (VSDs) and optical imaging are useful tools for studying spatiotemporal patterns of population neuronal activity in cortical tissue. Using a photodiode array and absorption dyes, we are able to record simultaneously from hundreds of points with sensitivity comparable to local field potential recordings. Since absorption dyes have less phototoxicity than fluorescent dyes, the total recording time in each slice can be 1,000–2,000 s, which can be divided into hundreds of short recording trials over several hours. In this chapter, we will discuss methodological details for achieving reliable VSD imaging with high sensitivity.
KeywordsShot Noise Local Field Potential Spiral Wave Amplitude Reduction Cortical Slice
This work was supported by NIH NS036477, NS059034, a Whitehall Foundation grant to JYW, and a fellowship grant from the American Epilepsy Society and Lennox Trust Fund to XH.
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