Monitoring Population Membrane Potential Signals from Neocortex

  • Xiaoying Huang
  • Weifeng Xu
  • Kentaroh Takagaki
  • Jian-Young Wu


Voltage-sensitive dyes (VSDs) and optical imaging are useful tools for studying spatiotemporal patterns of population neuronal activity in cortical tissue. Using a photodiode array and absorption dyes, we are able to record simultaneously from hundreds of points with sensitivity comparable to local field potential recordings. Since absorption dyes have less phototoxicity than fluorescent dyes, the total recording time in each slice can be 1,000–2,000 s, which can be divided into hundreds of short recording trials over several hours. In this chapter, we will discuss methodological details for achieving reliable VSD imaging with high sensitivity.


Shot Noise Local Field Potential Spiral Wave Amplitude Reduction Cortical Slice 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by NIH NS036477, NS059034, a Whitehall Foundation grant to JYW, and a fellowship grant from the American Epilepsy Society and Lennox Trust Fund to XH.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Xiaoying Huang
    • 1
  • Weifeng Xu
    • 1
  • Kentaroh Takagaki
    • 1
  • Jian-Young Wu
    • 1
  1. 1.Department of Physiology and BiophysicsGeorgetown University Medical CenterWashingtonUSA

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