Use of Fast-Responding Voltage-Sensitive Dyes for Large-Scale Recording of Neuronal Spiking Activity with Single-Cell Resolution
Optical recording with fast voltage sensitive dyes makes it possible, in suitable preparations, to simultaneously monitor the action potentials of large numbers of individual neurons. Here we describe methods for doing this, including considerations of different dyes and imaging systems, methods for correlating the optical signals with their source neurons, procedures for getting good signals, and the use of Independent Component Analysis for spike-sorting raw optical data into single neuron traces. These combined tools represent a powerful approach for large-scale recording of neural networks with high temporal and spatial resolution.
KeywordsIndependent Component Analysis Independent Component Analysis Optical Recording Spike Sorting Multiple Neuron
Supported by NS060921, Dart Foundation and Grass Foundation Marine Biological Laboratory summer fellowships, the Fred B. Snite Foundation, Rosalind Franklin University of Medicine and Science (WF), and the Howard Hughes Medical Institute (TS). We thank JY Wu, LB Cohen and L Eliot for comments on the manuscript.
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