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Use of Fast-Responding Voltage-Sensitive Dyes for Large-Scale Recording of Neuronal Spiking Activity with Single-Cell Resolution

  • William N. Frost
  • Jean Wang
  • Christopher J. Brandon
  • Caroline Moore-Kochlacs
  • Terrence J. Sejnowski
  • Evan S. Hill
Chapter

Abstract

Optical recording with fast voltage sensitive dyes makes it possible, in suitable preparations, to simultaneously monitor the action potentials of large numbers of individual neurons. Here we describe methods for doing this, including considerations of different dyes and imaging systems, methods for correlating the optical signals with their source neurons, procedures for getting good signals, and the use of Independent Component Analysis for spike-sorting raw optical data into single neuron traces. These combined tools represent a powerful approach for large-scale recording of neural networks with high temporal and spatial resolution.

Keywords

Independent Component Analysis Independent Component Analysis Optical Recording Spike Sorting Multiple Neuron 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

Supported by NS060921, Dart Foundation and Grass Foundation Marine Biological Laboratory summer fellowships, the Fred B. Snite Foundation, Rosalind Franklin University of Medicine and Science (WF), and the Howard Hughes Medical Institute (TS). We thank JY Wu, LB Cohen and L Eliot for comments on the manuscript.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • William N. Frost
    • 1
  • Jean Wang
    • 1
  • Christopher J. Brandon
    • 1
  • Caroline Moore-Kochlacs
    • 2
  • Terrence J. Sejnowski
    • 2
    • 3
  • Evan S. Hill
    • 1
  1. 1.Department of Cell Biology and Anatomy, The Chicago Medical SchoolRosalind Franklin University of Medicine and ScienceNorth ChicagoUSA
  2. 2.Howard Hughes Medical Institute, The Salk Institute for Biological StudiesLa JollaUSA
  3. 3.Division of Biological SciencesUniversity of California San DiegoLa JollaUSA

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