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Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines

  • Marco Canepari
  • Marko Popovic
  • Kaspar Vogt
  • Knut Holthoff
  • Arthur Konnerth
  • Brian M. Salzberg
  • Amiram Grinvald
  • Srdjan D. Antic
  • Dejan Zecevic
Chapter

Abstract

A central question in neuronal network analysis is how the interaction between individual neurons produces behavior and behavioral modifications. This task depends critically on how exactly are signals integrated by individual nerve cells functioning as complex operational units. Regional electrical properties of branching neuronal processes that determine the input–output function of any neuron are extraordinarily complex, dynamic, and, in the general case, impossible to predict in the absence of detailed measurements. To obtain such a measurement one would, ideally, like to be able to monitor, at multiple sites, subthreshold events as they travel from the sites of origin (synaptic contacts on distal dendrites) and summate at particular locations to influence action potential initiation. It became possible recently to carry out this type of measurement using high-resolution multisite recording of membrane potential changes with intracellular voltage-sensitive dyes. This chapter reviews the development and foundation of the msethod of voltage-sensitive dye recording from individual neurons. Presently, this approach allows monitoring membrane potential transients from all parts of the dendritic tree as well as from axon collaterals and individual dendritic spines.

Keywords

Fluorescence Resonance Energy Transfer Second Harmonic Generation Dendritic Spine Shot Noise Optical Recording 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Marco Canepari
    • 1
  • Marko Popovic
    • 2
  • Kaspar Vogt
    • 1
  • Knut Holthoff
    • 3
  • Arthur Konnerth
    • 4
  • Brian M. Salzberg
    • 5
  • Amiram Grinvald
    • 6
  • Srdjan D. Antic
    • 7
  • Dejan Zecevic
    • 2
  1. 1.Division of Pharmacology and NeurobiologyBiozentrum – University of BaselBaselSwiterland
  2. 2.Department of Cellular and Molecular PhysiologyYale University School of MedicineNew HavenUSA
  3. 3.Hans Berger Klinik für NeurologieFriedrich-Schiller-Universität JenaJenaGermany
  4. 4.Center for Intergrated Protein Science and Institute of NeuroscienceTechnical University MunichMunichGermany
  5. 5.Departments of Neuroscience and PhysiologyUniversity of Pennsylvania School of MedicinePhiladelphiaPennsylvania
  6. 6.Department of NeurobiologyWeizmann Institute of ScienceRehovotIsrael
  7. 7.Department of NeuroscienceUConn Health CenterFarmingtonUSA

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