Ancillary Studies in FNAC of Soft Tissue and Bone Lesions
Fine needle aspiration biopsy is useful in the diagnosis of bone and soft tissue tumors, especially for superficial primary sarcomas and metastatic or recurrent disease. The rinse of the biopsy needle often generates an adequate tissue for cell block preparation, which is a valuable source of specimen for ancillary studies. Ancillary studies generally include immunocytochemistry (IHC), cytogenetic karyotyping, flow cytometry, electron microscopy (EM), fluorescence in situ hybridization (FISH), reverse transcriptase polymerase chain reaction (RT-PCR), and mutational analysis. Karyotyping (will detect structural and numerical chromosomal abnormality when sarcoma is suspected, Table 9.1) and flow cytometry (will detect the clonality when lymphoma is suspected) require fresh tumor sample.
KeywordsReverse Transcriptase Polymerase Chain Reaction Synovial Sarcoma Anaplastic Large Cell Lymphoma Granular Cell Tumor Smooth Muscle Tumor
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