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Ecotoxicological Techniques and Assessment of Environmental Samples

Chapter

Abstract

In this chapter the primary aspect was to dissect and determine the toxic nature of effluent and environmental samples (river sediments and water). This also involved sample manipulation coupled to a biosensor toxicity assay for the purpose of identifying possible remediation strategies for future environmental conservancy. Traditionally, the tanning industry has been associated with odours and water pollution from partially or untreated discharges. Sparging was used to identify toxicity associated with volatile organic compounds. This type of ­toxicity testing technique was found to be ideal for samples collected from tannery ­effluent treatment pits or pans and anaerobic lagoons. For example the toxicity of ­contaminants removed by treatment with activated charcoal, was identified for all the sampling points (tannery effluent treatment pits, anaerobic lagoons and riverine sampling points) except for the points upstream. A similar result was observed when filtration, as a technique, was used to identify toxicity associated with suspended solids. The approach used highlighted the complex nature of the toxic ­pollutants in the tannery effluent. Moreover the results strongly indicated the polluting sources and also the possible remediation strategies for effluents at various stages of the tanning industry.

Keywords

Chemical Oxygen Demand Biological Oxygen Demand Ecological Risk Assessment Anaerobic Lagoon Double Deionise Water 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Alexander M (2000) Ageing, bioavailability, and overestimation of risk from environmental ­pollutants: critical review. Environ Toxicol Chem 34: 4259–4265.Google Scholar
  2. Allan-King RM, Baker JF, Gillham RW, Jensen BK (1994) Substrate and nutrient limited toluene biotransformation in sandy soil. Environ Toxicol Chem 13: 693–705.CrossRefGoogle Scholar
  3. Amin-Hanjani S, Meikle A, Glover LA, Prosser JI, Killham K (1993) Plasmid and chromosomally encoded luminescence marker systems for detection of Pseudomonas fluorescens in soil. Mol Ecol 2: 47–54.CrossRefGoogle Scholar
  4. Atlas RM, Bartha R (1993) Microbial Ecology. Fundamentals and Applications, 3rd edn. Benjamin/Cummings, Redwood City, CA, USA.Google Scholar
  5. Artiola JF (1996) Waste disposal, chapter 10. pp. 142. In: Pepper LI, Gerba PC, Brusseau ML (eds.) Pollution Science, Academic Press (Elsevier Science, USA), San Diego, California, USA.Google Scholar
  6. Brookes PC, Mcgrath SP, Klein DA, Elliot ET (1984) Effects of heavy metals on microbial activity and biomass in field soils treated with sewage sludge. In: Environmental Contamination (International Conference, London, July (1984), pp. 574–583, CEP, Edinburgh.Google Scholar
  7. Benefield CB, Haward PJA, Howard DM (1977) The estimation of dehydrogenase activity in soil. Soil Biol Biochem 9: 67–70.CrossRefGoogle Scholar
  8. Boyd EM, Meharg AA, Wright J, Killham K (1998) Toxicity of chlorobenzene to a lux-marked terrestrial bacterium, Pseudomonas flourescens. Environ Toxicol Chem 16: 849–856.Google Scholar
  9. Brown JS, Rattray EAS, Paton GI, Reid G, Caffoor I, Killham K (1996) Comparative assessment of the toxicity of a paper mill effluent by respiratory and luminescence based bacteria assay. Chemosphere 32: 1553–1561.CrossRefGoogle Scholar
  10. Burns RG (1978) Soil enzymes. Academic Press, New York.Google Scholar
  11. Cairns J Jr, Prat JR (1989) The scientific basis of bioassays. Hydrobiologia 189: 5–20.CrossRefGoogle Scholar
  12. Cervantes C, Campos-Garcia J, Devars S, Guitierrez-Corona F, Loza-Tavera H, Torres-Guzman JC, Moreno-Sanchez R (2000) Interactions of the chromium with microorganisms and plants. FEMS Microbiol Rev 25: 335–347.CrossRefGoogle Scholar
  13. Cenci H, Morozzi G (1979) The validity of the TTC-test for dehydrogenase activity of activated sludge in the presence of chemical inhibitors. Zentralblatt Bacteriol Hygiene Abstrat B 169: 320–330.Google Scholar
  14. Chaudri AM, Knight BP, Barbosa-Jefferson VL, Preston S, Paton GI, Killham K, Coad N, Nicholson FA, Chambers BJ, McGrath SP (1999) Determination of acute Zn toxicity in pore water from soils previously treated with sewage sludge using bioluminescence assays. Environ Sci Tech 33: 1880–1885.CrossRefGoogle Scholar
  15. Chen JM, Hao OJ (1998) Microbial chromium (VI) reduction. Crit Rev Environ Sci Tech 28: 219–251.CrossRefGoogle Scholar
  16. Chirwa EMN, Wang YT (1997) Chromium (VI) reduction by Pseudomonas fluorescens LB 300 in fixed-film bioreactor. J Environ Eng 123: 760–766.CrossRefGoogle Scholar
  17. Cronin MTD, Schultz TW (1997) Validation of Vibrio fischeri acute toxicity data: mechanism of action-based QSARs for non-polar narcotics and polar narcotic phenols. Sci Total Environ 204: 75–88.CrossRefGoogle Scholar
  18. Duncan S, Glover LA, Killham K, Prosser JI (1994) Luminescence-based detection of activity of starved and viable but non-culturable bacteria. Appl Environ Microbiol 60: 1308–1316.Google Scholar
  19. Doleman P, Haanstra L (1979) Effect of lead on soil respiration and dehydrogenase activity. Soil Biol Biochem 11: 475–479.CrossRefGoogle Scholar
  20. Escher BI, Snozzi M, Schwarzenbach RP (1996) Uptake, speciation and uncoupling activity of substituted phenols in energy transducing membranes. Environ Sci Tech 30: 3071–3079.CrossRefGoogle Scholar
  21. Gadd GM (1990) Metal tolerance pp. 178–210. In: Edwards C (ed.) Microbiology of Extreme Environments. Open University Press, Milton Keynes, UK.Google Scholar
  22. Galli R, Munz CD, Scholtz R (1994) Evaluation and application of aquatic toxicity tests: use of the Microtox test for the prediction of toxicity based upon concentrations of contaminants in the soil. Hydrobiologia 273: 179–189.CrossRefGoogle Scholar
  23. Gersberg RM, Carroquino MJ, Fisher DE, Dawsey J (1995) Biomonitoring of toxicity reduction during in situ bioremediation of monoaromatic compounds in groundwater. Water Res 29: 545–550.CrossRefGoogle Scholar
  24. Heitzer A, Webb OF, Thornnard JE, Sayer GS (1992) Specific and quantitative assessment of napthalene and salicylate bioavailability by using a bioluminescent catabolic reporter bacterium. Appl Environ Microbiol 58: 1839–1846.Google Scholar
  25. Hongwei Y, Zhanpeng J, Shaoqi S, Tang WZ (2002) INT-dehydrogenase activity test for assessing anaerobic biodegradability of organic compounds. Ecotoxicol Environ Saf 53: 416–421.CrossRefGoogle Scholar
  26. Knight B, McGrath SP, Killham K, Preston S, Paton G.I (1999) Assessment of the toxicity of heavy metals in soils amended with sewage sludge using a chemical speciation technique and a lux biosensor. Environ Toxicol Chem 18: 659–663.Google Scholar
  27. Knight BP, McGrath SP (1995) A method to buffer the concentrations of free Zn and Cd ions using a cation exchange resin in bacterial toxicity studies. Environ Toxicol Chem 14: (12): 2033–2039.CrossRefGoogle Scholar
  28. Killham K (2004) Personal communications.Google Scholar
  29. Kotas´ J, Stasicka Z (2000) Chromium occurrence in the environment and methods of its speciation. Environ Pollut 107: 263–283.CrossRefGoogle Scholar
  30. Lagido C, Pettit J, Porter AJR, Paton GI, Glover LA (2001) Development and application of bioluminescent Caenorhabditis elegans as multicellular eukaryotic biosensors. FEMS Microbiol Lett 493: 95–107.Google Scholar
  31. McGrath SP, Knight B, Killham K, Preston S, Paton GI (1999) Assessment of the toxicity of metals in soils amended with sewage sludge using a chemical speciation technique and a lux-based biosensor. Environ Toxicol Chem 18: 659–663.Google Scholar
  32. Mwinyihija, M., Strachan, N.J.C., Meharg, A. and Killham K (2005a) Biosensor based toxicity dissection of tannery and associated environmental samples. J Am Leather Chem Assoc 100: 381–490.Google Scholar
  33. Mwinyihija, M., Strachan, N.J.C., Meharg, A. and Killham K (2005b) Ecological risk assessment of the Kenyan tanning industry. J Am Leather Chem Assoc 100: 380–395.Google Scholar
  34. Mwinyihija M, Strachan NJ, Dawson J, Meharg A, and Killham K (2006) An Ecotoxicological approach to assessing the impact of tanning industry effluent on river health. Arch Environ Contam Toxicol 50: 316–324.CrossRefGoogle Scholar
  35. Okazaki, M., Hirata, E., and Tensho K (1983) TTC reduction in submerged soils. Soil Sci Plant Nutr 29: 489–497.CrossRefGoogle Scholar
  36. Packard TT (1971) The measurement of respiratory electron-transport activity in marine phytoplankton. J Mar Res 29: 235–245.Google Scholar
  37. Paton, G.I, Campbell, C.D., Glover, L.A., and Killham K (1995) Assessment of bioavailability of heavy metals using lux modified constructs of Pseudomonas fluorescens. Lett Appl Microbiol 20:52–56.CrossRefGoogle Scholar
  38. Paton GI, Rattary EAS, Campbell CD, Cressor MS, Glover LA, Meeussen JCL, Killham K (1997b) Use of genetically modified biosensors for soil ecotoxicity testing. pp. 397–418. In: Pankhurst C, Doube B, Gupta V (eds.) Biological indicators of Soil Health and Sustainable Productivity. CAB International, Oxford. Google Scholar
  39. Pflug W, Ziechman W (1982) Humic acids and the disruption of bacterial cell walls by lysozyme. Soil Biol. Biochem 14: 165–166.CrossRefGoogle Scholar
  40. Prasard BGS, Thyagarajan G, Nayudamma Y (1991) Utilisation of Water Hyacinth in the treatment and disposal of tannery wastewater. J Soc Leather Technol Chem 75: 56–57.Google Scholar
  41. Ritchie JM, Cresser M, Cotter-Howells (2001) Toxicological response of a bioluminescence microbial assay to Zn, Pb and Cu in artificial soil solution: relationship with total metal ­concentrations and free ion activities. Environ Pollut 144: 129–136.CrossRefGoogle Scholar
  42. Ros M, Ganter A (1998) Possibilities of reduction of recipient loading of tannery waste Slovenia. Water Sci Tech 37: 145–152.Google Scholar
  43. Ruhling A, Tyler G (1973) Heavy metal pollution and decomposition of spruce needle litter. Oikos 24: 402–416.CrossRefGoogle Scholar
  44. Sarin C (2000) A lux-based bioassay of heavy metal contamination of wastes. Ph.D Thesis, University of Aberdeen, UK.Google Scholar
  45. Saxena D, Levin R, Firer MA (2000) Removal of chromate from industrial effluent by a new isolate Staphylococcus chonii. Water Sci Tech 42: 93–98.Google Scholar
  46. Schinner FA, Niederbacher R, Neuwinger I (1980) Influence of compound fertiliser and cupric sulphate on soil enzymes and CO2 evolution. Plant Soil 57: 85–93.CrossRefGoogle Scholar
  47. Shaw LJY, Glover LA, Killham K, Osborn D, Meharg AA (2000) Bioavailability of 2,4-­dichlorophenol associated with soil water-soluble humic material. Environ Sci Tech 34: 4721–4726.CrossRefGoogle Scholar
  48. Shen H, Wang YT (1993) Characterisation of enzymatic reduction of hexavalent chromium by Escherichia coli ATCC 33456. Appl Environ Microbiol 59: 3771–3777.Google Scholar
  49. Sinclair MG (1999) Soil toxicity assessment of 2,4-DCP using lux microbial biosensors. PhD thesis, University of Aberdeen, U.K.Google Scholar
  50. Skujins J (1978) History of abiotic soil enzyme research. pp. 1–49. In: Burns RG (ed.) Soil Enzymes. Academic Press, New York.Google Scholar
  51. Sommervile L, Greaves MP, Verstraete W, Poole NJ, van Dijk H, Anderson JPE (1978) Recommended laboratory tests for assessing the side effects of pesticides in soil microflora. In: Sommerville L, Greaves MP (eds.) Pesticide effects on soil microflora, Taylor and Francis, London.Google Scholar
  52. Song Z, Williams CJ, Edyvean RJ (2000) Sedimentation of tannery wastewater. Water Res 34: 2171–2176.CrossRefGoogle Scholar
  53. Sousa S, Duffy C, Weitz H, Glover AL, Bar E, Henkler R, Killham K (1998) Use of a lux-modified bacterial biosensor to identify constraints to bioremediation of BTEX-contaminated sites. Environ Toxicol Chem 17: 1039–1045.Google Scholar
  54. Sposito G (1989) The chemistry of soils. Oxford University Press, LondonGoogle Scholar
  55. Suter GW (1993) Ecological risk assessment. Lewis Publishers, Boca Raton, Florida, USA.Google Scholar
  56. Stein K, Schwedt G (1994) Chromium speciation in the wastewater from a tannery. Fresen J Anal Chem 350: 38–41.CrossRefGoogle Scholar
  57. Steinberg SM, Poziomek EJ, Engelman WH, Rogers KR (1995) A review of environmental applications of bioluminescence measurements. Chemosphere 30: 2155–2195.CrossRefGoogle Scholar
  58. Stuhlfauth T (1995) Ecotoxicological monitoring of industrial effluents, chapter 14 pp. 187. In: Richardson M (ed.) Environmental toxicology assessment. Taylor & Francis, Hertfordshire, United Kingdom.Google Scholar
  59. Thanikaivelan P, Rao RJ, Nair BU, Ramasami T (2003) Approach towards zero discharge tanning: role of concentration on the development of eco-friendly liming-reliming processes. J Clean Prod 11: 79–90.CrossRefGoogle Scholar
  60. Trevors JT, Mayfield CI, Innis WE (1981) A rapid toxicty test using Pseudomonas fluorescens. Bull Environ Contam Toxicol 26: 433–437.CrossRefGoogle Scholar
  61. UK Habitat directives (2004) 3rd revision of habitats directives and regulations guidance. www.ennvironment-agency.gov.uk. Accessed on 22nd April 2005
  62. USEPA (U.S. Environmental Protection Agency) (1991) Methods for aquatic toxicity identification evaluations: Phase I toxicity characterisation procedures. 2nd Edition EPA/600/6-91/003. Environmental Research Laboratory, Duluth, MN, USA.Google Scholar
  63. UNEP IE/PAC (1994) Tanneries and the Environment – A Technical Guide, Technical Report (2nd Print) Series No 4, ISBN 92 807 1276 4.Google Scholar
  64. van Leeuwen HP (1999) Metal speciation dynamics and bioavailability: inert and labile complexes. Environ Sci Tech 33: 3734–3748.Google Scholar
  65. Walsh AR, O’Halloran J (1996a) Chromium speciation in tannery effluent – 1: an assessment of techniques and role of organic Cr(III) complexes. Water Res 30: 2393–2400.CrossRefGoogle Scholar
  66. Walsh AR, O’Halloran J (1996b) Chromium speciation in Tannery effluents – II: Speciation in effluent and in a receiving estuary. Water Res 30: 2401–2412.CrossRefGoogle Scholar
  67. Wararatananurak P (2000) Fractionation of chromium toxicity in water. PhD Thesis, University of Aberdeen, U.K.Google Scholar
  68. Wild SR, Harrad SJ, Jones K (1993) Chlorophenols in digested UK sewage sludges. Water Res 27: 1527–1534.CrossRefGoogle Scholar

Copyright information

© Springer New York 2010

Authors and Affiliations

  1. 1.Leather Development CouncilNairobiKenya

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