Spectrophotometry and Optical Biosensor

  • Jeong-Yeol Yoon


In the previous chapter we learned about optoelectronic light sensors, especially photodiode. Although many photodiodes are made to be specifically sensitive to a certain range of colors, e.g., UV-blue, red-IR, etc., they cannot tell the exact color or color combination of light signal. Photodiodes (and most other optoelectronic light sensors) simply inform us the intensity of light signals. Detailed information on color combination can be obtained by using a technique known as spectrophotometry, which involves an instrument spectrophotometer. Spectrophotometers are quite bulky and relatively expensive and have not been considered as a topic for sensors or biosensors. Recent years, however, very small spectrophotometers (a size comparable to a mobile phone) have become commercially available. In addition, spectrophotometers are becoming an integral part of many sensor and biosensor devices.


Pulse Oximeter Gluconic Acid Glucose Sensor Electrochemical Biosensor Laboratory Task 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



  1. Christian GD (2004) Analytical chemistry, 6th edn. Hoboken, WileyGoogle Scholar
  2. Cooper J, Cass T (eds) (2004) Biosensors, 2nd edn. Oxford University Press, OxfordGoogle Scholar
  3. Eggins BR (2002) Chemical sensors and biosensors. Wiley, West SussexGoogle Scholar
  4. Holme DJ, Peck H (1998) Analytical biochemistry, 3rd edn. Pearson Education, EssexGoogle Scholar
  5. Kissinger P (2005) Biosensors—a perspective. Biosens Bioelectron 20:2512–2516CrossRefGoogle Scholar
  6. Koschwanez H, Reichert W (2007) In vitro, in vivo and post explantation testing of glucose-detecting biosensors: Current methods and recommendations. Biomaterials 28:3687–3703CrossRefGoogle Scholar
  7. Skoog DA, Holler FJ, Nieman TA (2006) Principles of instrumental analysis, 6th edn. Saunders College, PhiladelphiaGoogle Scholar

Miniature Spectrometer (Section 8.2)

  1. Ocean Optics (2008) USB4000 fiber optic spectrometer installation and operation manual.
  2. USB4000operatinginstructions.pdf. p. 21. [Fig. 8.5]Google Scholar
  3. Ocean Optics (2012) CHEMUSB4-UV–VIS spectrophotometerGoogle Scholar

Optical glucose sensor (Section 8.3)

  1. Harborn U, Xie B, Venkatesh R, Danielsson B (1997) Evaluation of a miniaturized thermal biosensor for the determination of glucose in whole blood. Clin Chim Acta 267:225–237CrossRefGoogle Scholar
  2. Luong JHT, Male KB, Glennon JD (2008) Biosensor technology: technology push versus market pull. Biotechnol Adv 26:492–500CrossRefGoogle Scholar
  3. Newman J, Turner A (2005) Home blood glucose biosensors: a commercial perspective. Biosens Bioelectron 20:2435–2453. [Fig. 8.7]Google Scholar

Pulse Oximeter (Further Study)

  1. Bowes WA III, Corke BC (1989) Pulse oximetry: a review of the theory, accuracy, and clinical applications. Obstet Gynecol 74:541–546Google Scholar
  2. Mendelson Y (1992) Pulse oximetry: theory and applications for noninvasive monitoring. Clin Chem 38:1601–1607Google Scholar
  3. Tremper KK, Barker SJ (1989) Pulse oximetry. Anesthesiology 70:98–108CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Jeong-Yeol Yoon
    • 1
  1. 1.Department of Agricultural and Biosystems Engineering Department of Biomedical Engineering BIO5 InstituteUniversity of ArizonaTucsonUSA

Personalised recommendations